Enzymatic assembly of DNA molecules up to several hundred kilobases. (Record no. 52759)

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control field MX-MdCICY
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control field 20250625162415.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18612
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Title Enzymatic assembly of DNA molecules up to several hundred kilobases.
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Volume/sequential designation Nature methods, 6(5), p.343-345, 2009
520 3# - SUMMARY, ETC.
Summary, etc. We describe an isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5? exonuclease, a DNA polymerase and a DNA ligase. First we recessed DNA fragments, yielding single-stranded DNA overhangs that specifically annealed, and then covalently joined them. This assembly method can be used to seamlessly construct synthetic and natural genes, genetic pathways and entire genomes, and could be a useful molecular engineering tool.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DNA MOLECULES
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gibson, D. G.
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Personal name Young, L.
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Personal name Chuang, R. Y.
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Personal name Venter, J. C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hutchison, C. A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Smith, H. O.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/12K9Z54da4GzV2cDBdQcdGZN4h0CAxgM2/view?usp=drivesdk">https://drive.google.com/file/d/12K9Z54da4GzV2cDBdQcdGZN4h0CAxgM2/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18612 25.06.2025 25.06.2025 Documentos solicitados