Auxin and cytokinin mediated regulation involved in vitro organogenesis of papaya. (Record no. 52789)

MARC details
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fixed length control field 02566nam a2200301Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162416.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18643
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245 10 - TITLE STATEMENT
Title Auxin and cytokinin mediated regulation involved in vitro organogenesis of papaya.
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Plant Physiology, 260, p.153405., 2021
520 3# - SUMMARY, ETC.
Summary, etc. In vitro organogenesis is a multistep process which is largely controlled by the balance between auxin and cytokinin. Previous studies revealed a complex network regulating in vitro organogenesis in Arabidopsis thaliana; however, our knowledge of the molecular mechanisms underlying de novo shoot formation in papaya (Carica papaya)remains limited. Here, we optimized multiple factors to achieve an efficient and reproducible protocol for the induction of papaya callus formation and shoot regeneration. Subsequently, we analyzed the dynamic transcriptome profiles of samples undergoing this process, identified 5381, 642, 4047, and 2386 differentially expressed genes (DEGs), including 447, 66, 350, and 263 encoding transcription factors (TFs), in four stage comparisons. The DEGs were mainly involved in phytohormone modulation and transduction processes, particularly for auxin and cytokinin. Of these, 21 and 7 candidate genes involved in the auxin and cytokinin pathways, respectively, had distinct expression patterns throughout in vitro organogenesis. Furthermore, we found two genes encoding key TFs, CpLBD19 and CpESR1, were sharply induced on callus induction medium and shoot induction medium, indicating these two TFs may serve as proxies for callus induction and shoot formation in papaya. We therefore report a regulatory network of auxin and cytokinin signaling in papaya according to the one previously modeled for Arabidopsis. Our comprehensive analyses provide insight into the early molecular regulation of callus initiation and shoot formation in papaya, and are useful for the further identification of the regulators governing in vitro organogenesis.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element IN VITRO ORGANOGÉNESIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AUXIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CYTOKININ
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PAPAYA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSCRIPTOME
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhao, X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Song, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zeng, Q.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ma, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Fang, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yang, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yue, J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1Ks0qEg9C18ojbhCWoK3ZGi9QOEvWMrpV/view?usp=drivesdk">https://drive.google.com/file/d/1Ks0qEg9C18ojbhCWoK3ZGi9QOEvWMrpV/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18643 25.06.2025 25.06.2025 Documentos solicitados