MARC details
| 000 -LEADER |
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| fixed length control field |
02466nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162416.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-18646 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
CRISPR/Cas9-mediated genome editing in Hevea brasiliensis. |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Industrial Crops and Products, 164, p.113418., 2021 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Clustered regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9 (CRISPR/Cas9)has not been completely established in Hevea brasiliensis. In the present study, firstly, five endogenous U6 promoters from H. brasiliensis were identified and exploited to drive single guide RNA (sgRNA)transcription for establishing CRISPR/Cas9 transient editing system in H. brasiliensis protoplast. All five promoters were functional, however, their corresponding editing efficiencies were different and varied from 8.47 percent to 24.92 percent. Secondly, mutation profiles were characterized using 10 sgRNAs targeted five flowering time related genes (HbFT1, HbFT2 and HbTFL1?1, HbTFL1?2, HbTFL1?3)in H. brasiliensis protoplast. Three mutation patterns i. e. deletion, insertion and base substitution were detected. Among these mutations, deletion was the most prominent one and the insertions were observed only in half of 10 target sites, in which the highest insertion frequency (15 percent)occurred at TS6 target site. Base substitutions including transition and transversion were detected in 8 out of 10 target sites by deep sequencing. Lastly, stable transformation editing vector targeting phytoene desaturase gene (HbPDS)was constructed and transformed into rubber tree callus, and the "+1" bp homozygous insertions were detected for 3 out of 16 calli at the target site, which turned to expected albino phenotype in the following subculture. This study pronounced the establishment of genome editing in H. brasiliensis by CRISPR/Cas9 plasmid system. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HEVEA BRASILIENSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CRISPR/CAS9 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HBU6 PROMOTER |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GENOME EDITING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PROTOPLAST |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CALLUS |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Dai, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Yang, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wang, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Fan, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Xin, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Hua, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Huang, H. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1utBil-b7uiZPy9b_6bn05wu_eWk2b_sc/view?usp=drivesdk">https://drive.google.com/file/d/1utBil-b7uiZPy9b_6bn05wu_eWk2b_sc/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
