Expression of key auxin biosynthesis genes correlates with auxin and starch content of developing wheat (Triticum aestivum)grains. (Record no. 52793)

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fixed length control field 02374nam a2200313Ia 4500
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control field MX-MdCICY
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control field 20250625162416.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18647
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Title Expression of key auxin biosynthesis genes correlates with auxin and starch content of developing wheat (Triticum aestivum)grains.
490 0# - SERIES STATEMENT
Volume/sequential designation Functional Plant Biology, doi: 10.1071/FP20319, 2021
520 3# - SUMMARY, ETC.
Summary, etc. The effect of auxin on wheat (Triticum aestivum L.)grain size is contentious. Additionally, the contributions to the IAA pool from de novo synthesis versus hydrolysis of IAA-glucose are unclear. Here, we describe the first comprehensive study of tryptophan aminotransferase and indole-3-pyruvate mono-oxygenase expression from 5 to 20 days after anthesis. A comparison of expression data with measurements of endogenous IAA via combined liquid chromatography-tandem mass spectrometry using heavy isotope labelled internal standards indicates that TaTAR2-B3, TaYUC9-A1, TaYUC9-B, TaYUC9-D1, TaYUC10-A and TaYUC10-D are primarily responsible for IAA production in developing grains. Furthermore, these genes are expressed specifically in developing grains, like those found in rice (Oryza sativa L.)and maize (Zea mays L.). Our results cast doubt on the proposed role of THOUSAND-GRAIN WEIGHT gene, TaTGW6, in promoting larger grain size via negative effects on grain IAA content. Work on this gene overlooked the contribution of IAA biosynthesis from tryptophan. Although IAA synthesis occurs primarily in the endosperm, we show the TaYUC9-1 group is also strongly expressed in the embryo. Within the endosperm, TaYUC9-1 expression is highest in aleurone and transfer cells, suggesting that IAA has a key role in differentiation of these tissues as has been proposed for other cereals.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AUXIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GRAIN FILL
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Topical term or geographic name entry element TATAR2
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TAYUC
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TATGW6
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TATGW-7ª
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRITICUM AESTIVUM L.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GRAIN DEVELOPMENT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GRAIN SIZE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kabir, M. R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Nonhebel, H. M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Backhouse, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Winter, G.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1ifbllWKKtC7CGMsIIm5f_PH2vg_qYxQ8/view?usp=drivesdk">https://drive.google.com/file/d/1ifbllWKKtC7CGMsIIm5f_PH2vg_qYxQ8/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18647 25.06.2025 25.06.2025 Documentos solicitados