Direct somatic embryogenesis using leaf explants and short term storage of synseeds in Spathoglottis plicata Blume. (Record no. 52799)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02638nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162416.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-18653 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Direct somatic embryogenesis using leaf explants and short term storage of synseeds in Spathoglottis plicata Blume. |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Plant Cell, Tissue and Organ Culture , 145(2), p.321-331, 2021 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Spathoglottis plicata Blume is a vulnerable orchid species in various parts of the world, and the conventional propagation provides limited success to its cultivation and conservation. Therefore, present study deals with the direct induction of somatic embryos (SEs)from the leaf explants of S. plicata. Murashige and Skoog's (MS)medium fortified with various types and concentrations of plant growth regulators were used to induce somatic embryogenesis and plantlet production. The highest percentage of somatic embryo formation (93.7?±?0.56 percent)was achieved on MS medium supplemented with 1.0 mg L?1 2,4-dichlorophenoxyacetic acid (2,4-D), whereas, maximum proliferation and increase in fresh weight (FW)of SEs (149.5?±?0.24 mg/ 50 mg initial FW)was achieved on MS medium fortified with 2.0 mg L?1 6-benzylaminopurine (BAP)within 5 weeks of incubation. The light microscopic observation revealed that SEs emerged directly from the leaf surface. The viable synthetic seeds (SS)from SEs were prepared by encapsulating with gel matrix of 3 percent (w/v)sodium alginate and 100 mM calcium chloride. The SS were successfully stored for 60 days at ??4?°C with 97.4 percent germination frequency and shoot proliferation using MS medium with 2.0 mg L?1 BAP and 0.25 mg L?1 indole-3-acetic acid. The synergistic rooting frequency was observed on half-strength MS medium with 1.0 mg L?1 indole-3-butyric acid. The rooted shoots were acclimatized in a greenhouse with a 90 percent survival rate using soilrite® and vermicompost. This is an efficient short-term storage and regeneration protocol for S. plicata, which could help in reducing pressure on its wild population and could also be extended to the cryopreservation of this orchid species. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ARTIFCIAL SEDES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | COLD STORAGE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | IN VITRO REGENERATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | LIGHT MICROSCOPY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SPATHOGLOTTIS PLICATA |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Manokari, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Priyadharshini, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Shekhawat, M. S. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1kYxoJhvH1ckSkrmHjpuKUTudoIIULhet/view?usp=drivesdk">https://drive.google.com/file/d/1kYxoJhvH1ckSkrmHjpuKUTudoIIULhet/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-18653 | 25.06.2025 | 25.06.2025 | Documentos solicitados |