Agitation effect on growth and metabolic behavior of plant cell suspension cultures of Thevetia peruviana at bench scale reactor. (Record no. 52800)

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fixed length control field 02337nam a2200253Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162416.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18654
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title Agitation effect on growth and metabolic behavior of plant cell suspension cultures of Thevetia peruviana at bench scale reactor.
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Cell, Tissue and Organ Culture , 145(2), p.307-319, 2021
520 3# - SUMMARY, ETC.
Summary, etc. Plant cell suspension cultures of Thevetia peruviana has been explored for pharmaceutical compounds production. The aim of this study was to evaluate the agitation rate effect on growth and metabolism behavior of T. peruviana cells grown in a 7 L stirred tank reactor. Increases in agitation rates favored cell growth, secondary metabolites production and metabolic activity. The highest biomass concentration 11.92?±?0.25 g DW/L was reached at 550 rpm. The oxidase-reductase activity was stimulated at 550 and 800 rpm. Guaiacol peroxidase activity showed an increase for 300 and 550 rpm after day 7. High levels of extracellular Reactive Oxygen Species (ROS)were observed at day 7 for 550 and 800 rpm. Intracellular phenolic compounds (PC)showed an upward trend until day 7 with a maximum phenolic production of 57.78?±?4.70 mg EGA/100 g FW for 550 rpm. These results indicated that cells responded to ROS stress in a non-enzymatic manner during the first 7 days of culture, increasing PC production with antioxidant capacity. After 7 days, cells responded enzymatically. Intracellullar cardiac glycoside showed a relative increase of 1.7 and 2.1 times for 550 and 800 rpm, respectively. The maximum extracellular production of cardiac glycoside for 550 and 800 rpm was 770.34?±?42.84 mg EP/L. Taken together this study established reactor culture conditions for production of cardiac glycosides and PC, especially taxifolin.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element THEVETIA PERUVIANA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLANT CELL REACTOR CULTURES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AGITATION EFECTS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element METABOLIC ACTIVITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SECONDARY METABOLITES
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Arias, J. P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mendoza, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Arias, M.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1UujMAma3AQ9Ht0mV4RpIcZqfG4Cqmzli/view?usp=drivesdk">https://drive.google.com/file/d/1UujMAma3AQ9Ht0mV4RpIcZqfG4Cqmzli/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18654 25.06.2025 25.06.2025 Documentos solicitados