The pST44 polycistronic expression system for producing protein complexes in Escherichia coli. (Record no. 52809)

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fixed length control field 02015nam a2200253Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162416.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18663
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245 10 - TITLE STATEMENT
Title The pST44 polycistronic expression system for producing protein complexes in Escherichia coli.
490 0# - SERIES STATEMENT
Volume/sequential designation Protein expression and purification, 40(2), p.385-395, 2005
520 3# - SUMMARY, ETC.
Summary, etc. Protein complexes are responsible for key biological processes, but methods to produce recombinant protein complexes for biochemical and biophysical studies are limited. We have developed a second generation Escherichia coli polycistronic expression system which improves on the modularity of our original pST39 polycistronic system. This pST44 expression system simplifies the construction of polycis-tronic plasmids, particularly of variant plasmids expressing deletion or point mutations in any subunit. To facilitate purification of the expressed complex, we have prepared a suite of 72 plasmids which allows individual subunits to be tagged at the N- or C-terminus with six permanent or cleavable peptide affinity tags. We demonstrate these new features in a detailed deletion analysis of a three protein yeast Piccolo NuA4 histone acetyltransferase complex, and in the affinity purification of a human Piccolo NuA4 complex. We also utilize the modular design to show that the order of expression of the three subunits along the polycistronic plasmid does not affect the reconstitution of the yeast Piccolo complex in E. coli.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element POLYCISTRONIC EXPRESSION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element COEXPRESSION OF PROTEIN COMPLEXES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AFFINITY TAGS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HISTONE ACETYLTRANSFERASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RECOMBINANT EXPRESSION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tan, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kern, R. C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Selleck, W.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1Kc7Hf3-z7gb1HgFlVxbrmJVWsZ3-YEaN/view?usp=drivesdk">https://drive.google.com/file/d/1Kc7Hf3-z7gb1HgFlVxbrmJVWsZ3-YEaN/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18663 25.06.2025 25.06.2025 Documentos solicitados