Identification of key genes involved in catechin metabolism in tea seedlings based on transcriptomic and HPLC analysis (Record no. 52860)

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control field 20250625162417.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18714
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Title Identification of key genes involved in catechin metabolism in tea seedlings based on transcriptomic and HPLC analysis
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Volume/sequential designation Plant Physiology and BioChemistry, 133, p.107-115, 2018
520 3# - SUMMARY, ETC.
Summary, etc. Tea is a non-alcoholic beverage with many benefits to human health and thereby widely consumed in the world. It contains plenty of secondary metabolites and tea catechins are the characteristic compounds. To further elucidate the biosynthetic and regulatory mechanisms of catechins in tea, high performance liquid chromatography (HPLC)and transcriptome analysis were performed in tea seedlings of different growth stages. A combined method of differential expression and correlation analysis was then conducted. The results showed that the order of total catechin (TC)contents was leaves > stems > roots, irrespective of growth stages. For transcriptome analysis, a total of 355.81 million clean reads were generated and mapped to the referencing tea genome. Further real time PCR analysis of 18 selected genes confirmed RNA-Seq results. A total of 7 structural genes and 35 transcription factors (TFs)were identified to be significantly correlated with TC changes. Among them, three TFs homologous toANL2,WRKY44andAtMYB113 might play key roles in catechin regulation. The de novotranscriptome data of different organs in tea seedlings provided new insights into the biosynthetic and metabolic pathways of catechins.
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Topical term or geographic name entry element CAMELLIA SINENSIS
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Topical term or geographic name entry element CORRELATION ANALYSIS
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Topical term or geographic name entry element TRANSCRIPTOME ANALYSIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CATECHIN BIOSYNTHESIS
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Topical term or geographic name entry element TRANSCRIPTION FACTOR
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Personal name Zhang, Yazhen
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Personal name Wei, Kang
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Personal name Li, Hailin
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wang, Liyuan
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ruan, Li
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Pang, Dandan
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Cheng, Hao
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1hiXvswVoSZRjRYKvIJwRX-jMllgDyVJZ/view?usp=drivesdk">https://drive.google.com/file/d/1hiXvswVoSZRjRYKvIJwRX-jMllgDyVJZ/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18714 25.06.2025 25.06.2025 Documentos solicitados