MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02741nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20250625162418.0 |
| 040 ## - CATALOGING SOURCE |
|---|
| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-18742 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Ploidy level, epigenetic and in vitro environment infuence the indirect somatic embryogenesis of the new synthetic autoallohexaploid Cofea |
| 490 0# - SERIES STATEMENT |
|---|
| Volume/sequential designation |
Plant Cell, Tissue and Organ Culture, p.1-11, 2021 |
| 520 3# - SUMMARY, ETC. |
|---|
| Summary, etc. |
Autoallohexaploid plantlets of "Híbrido de Timor" (HT, Cofea)were recently regenerated by chromosome set doubling from allotriploid HT. Besides the in vitro propagation and chromosome doubling set, indirect somatic embryogenesis allows us to investigate the short-term polyploidy consequences. We aimed to establish and compare the indirect somatic embryogenesis in natural allotriploid and synthetic autoallohexaploid HT, evaluating the role of the in vitro environment, ploidy level and global level of 5-methylcytosine on somatic embryo regeneration and karyotype stability. Our results showed that the autoallohexaploid needed more time for induction and proliferation of friable calli, showing lower mean numbers of responsive explants and a higher mean level of 5-methylcytosine. Autoallohexaploid also showed a higher 5-methylcytosine level during the somatic embryo regeneration, but both hybrids exhibited the same mean value of mature cotyledonary somatic embryos for friable callus. Regarding the in vitro environment, the activated charcoal at 8 and 16 g L-1 increased global level of 5-methylcytosine and the mean number of abnormal somatic embryo in autoallohexaploid. Based on these results, the autoallohexaploid exhibited a lower in vitro response compared to its allotriploid ancestor, despite having at least twice as many copies of in vitro response genes. Besides, our data evidenced that the in vitro environment, ploidy level and global 5-methylcytosine level infuence the Cofeaindirect somatic embryogenesis, showing the outcome of the short-term induced polyploidy. Therefore, we showed the euploidy infuence during indirect somatic embryogenesis, demonstrating the relevance of the "omics" evaluations of the new Cofeagermplasm in relation to their ancestor. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
COFEE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
IN VITRO PROPAGATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
POLYPLOIDY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
PLANT TISSUE CULTURE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
EPIGENETIC |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Morais Oliveira, João Paulo De |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Arruda Sanglard, Natália |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ferreira, Adésio |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ronildo Clarindo, Wellington |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/13yzGW2mtdePaks839xVHeEmOHMcxribk/view?usp=drivesdk">https://drive.google.com/file/d/13yzGW2mtdePaks839xVHeEmOHMcxribk/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
