Comparative ontogenesis of Coffea arabica L. somatic embryos reveals the efficiency of regeneration modulated by the explant source and the embryogenesis pathway (Record no. 52951)

MARC details
000 -LEADER
fixed length control field 02562nam a2200301Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162419.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18805
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Comparative ontogenesis of Coffea arabica L. somatic embryos reveals the efficiency of regeneration modulated by the explant source and the embryogenesis pathway
490 0# - SERIES STATEMENT
Volume/sequential designation In Vitro Cellular & Developmental Biology-Plant, https://doi.org/10.1007/s11627-021-10200-5, p.1-15, 2021
520 3# - SUMMARY, ETC.
Summary, etc. In vitro micropropagation is an important technique for coffeemultiplication performed by somatic embryogenesis, which can be indirect, with the formation of calluses, or direct, when embryos are formed directly from explant cells. In the present study, we characterized the ontogenesis of somatic embryos regenerated via indirect and direct somatic embryogenesis in leaf explants from Coffea arabica 'Mundo Novo' and assessed the development of these embryos in leaf explants from adult plants maintained ex situ and in vitro. Anatomical analyses showed that leaf explants present structural differences depending on their origin. In the direct pathway, tissue develops more rapidly in explants from in vitro plants than in those from ex situ explants. In both methods, the formation of a pro-embryogenic mass was found to be essential for embryo formation. In the indirect pathway, calluses from ex situ material presented elongated cells and a loose external appearance. Conversely, calluses from in vitro material presented large regions of meristematic cells with a single large nucleus and dense cytoplasm. We propose that indirect somatic embryogenesis in in vitro-grown explants may accelerate genetic breeding in coffee, and it represents the most efficient condition for somatic embryogenesis, producing embryos in less time than the required by ex situ plants (indirect: 260 d in ex situ conditions, 62 d in vitro; direct: 270 d ex situ, 86 d in vitro).
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ANATOMY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CALLUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MICROPROPAGATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLANT GROWTH REGULATORS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PRO-EMBRYOGENICMASS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ferrari, I. F.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Marques, G. A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Junior, W. L. S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Biazotti, B. B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Passos, M. P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name De Almeida, J. A. S.,
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mayer, J. L. S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1y6_BmlaujDA_xgWAi-dJnpH1GXPLc44C/view?usp=drivesdk">https://drive.google.com/file/d/1y6_BmlaujDA_xgWAi-dJnpH1GXPLc44C/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18805 25.06.2025 25.06.2025 Documentos solicitados