MARC details
| 000 -LEADER |
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| fixed length control field |
02568nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162419.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-18807 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Study of the amount of oxidative damage to mitochondrial and chloroplast DNA in clones of white poplar (Populus alba L.)during long-term in vitro cultivation for 26 years |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Plant Molecular Biology, 106, p.479-489, 2021 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
In vitro conservation technology is used to preserve the valuable gene pool of forest woody plants. It is important to maintain genetic stability of the collection material. However, in the course of long-term subculturing, genetic variability of cells and tissues can accumulate, and morphological signs of culture aging can appear. It is likely that this is due to the use of various hormones in nutrient media. We have been supporting in vitro cultures of diploid and triploid clones of white poplar (Populus alba L.)in a hormone-free media for 26 years. We show that in 26-year-old clones, in comparison with 5-year-old clones, the amount of oxidative damage to mitochondrial DNA (mtDNA)and chloroplast DNA (chDNA)increases. In addition, there is an age-related decrease in the number of chDNA copies. Despite this, 26-year-old poplar clones retain regenerative activity, the ability of microshoot rooting, and normal growth, and there were no visible markers of ontogenetic aging. The clones also retained the ploidy typical for the mother tree. We hypothesize that the absence of phenotypic manifestations of mtDNA and chDNA damage is associated with optimal cultivation conditions, while stressful conditions could result in these damages. Thus, we show that the measurement of the amount of damage in non-nuclear DNA, using long-range PCR, can be a convenient method for assessing the senescing of clones in vitro during long-term cultivation; this approach is more sensitive and more accurate than morphological and cytogenetic methods. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
WHITE POPLAR |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MITOCHONDRIAL DNA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CHLOROPLAST DNA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
LONG-TERM IN VITRO CULTIVATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PLANT AGING |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Gureev, A. P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Mashkina, O. S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Shabanova, E. A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Vitkalova, I. Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Sitnikov, V. V. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Popov, V. N. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1Ip79rJkL0Ff4Ongm4-wLHgToGF9pIcw_/view?usp=drivesdk">https://drive.google.com/file/d/1Ip79rJkL0Ff4Ongm4-wLHgToGF9pIcw_/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
