Chloroplast transformation in oilseed rape (Record no. 53033)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01888nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162421.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-18890 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Chloroplast transformation in oilseed rape |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Transgenic Research, 12(1), p.111-114, 2003 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The chloroplast transformation vector pNRAB carries two expression cassettes for the spectinomycin resistance gene aadA and the insect resistance gene cry1Aa10. The two cassettes are sited between the rps7 and ndhB targeting fragments. Biolistic delivery of the vector DNA, followed by spectinomycin selection, yielded chloroplast transformants at a frequency of four in 1000 bombarded cotyledon petioles. PCR analysis and Southern blot of PCR products confirmed the site-specific integration of aadA and cry1Aa10 into the chloroplast genomes of transgenic oilseed rape. When transgenic oilseed rape leaves were fed to second instar Plutella xylostera larvae, 47 percent mortality was observed against this insect and the surviving larvae had significantly lower weight than the control. This is the first report of chloroplast transformation in oilseed rape and the introduction of novel genes between the rps7 and ndhB genes in the chloroplast genome. This offers an opportunity for improvement of oilseed rape by chloroplast genetic engineering. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CHLOROPLAST TRANSFORMATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | OILSEED RAPE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SITE-SPECIFIC INTEGRATION |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hou, B. K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhou, Y. H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wan, L. H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhang, Z. L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Shen, G. F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chen, Z. H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hu, Z. M |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1Y5lb4Su_-reKnp8GbosKLccf-hEFQvq-/view?usp=drivesdk">https://drive.google.com/file/d/1Y5lb4Su_-reKnp8GbosKLccf-hEFQvq-/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-18890 | 25.06.2025 | 25.06.2025 | Documentos solicitados |