Construction of an infectious clone of a plant RNA virus in a binary vector using one-step Gibson Assembly (Record no. 53088)

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000 -LEADER
fixed length control field 01579nam a2200229Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162422.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18945
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245 10 - TITLE STATEMENT
Title Construction of an infectious clone of a plant RNA virus in a binary vector using one-step Gibson Assembly
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of virological method, 222, p.11-15, 2015
520 3# - SUMMARY, ETC.
Summary, etc. The construction of full-length infectious clones of RNA viruses is often laborious due to the many cloning steps required and the DNA exclusion within the plasmid during Escherichia coli transformation. We demonstrate single-step cloning procedure of an infectious cDNA of the tomato blistering mosaic virus (ToBMV)using Gibson Assembly (GA), which drastically reduces the number of cloning steps. By agro-inoculation with the construct obtained by this procedure, ToBMV was recovered six days post-inoculation in Nicotiana benthamiana plants. The symptoms induced by the recovered virus were indistinguishable from those caused by the wild-type virus. We conclude that the GA is very useful method particularly to construct a full-length cDNA clone of a plant RNA virus in a binary vector.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FULL-LENGTH CDNA CLONE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GIBSON ASSEMBLY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SEAMLESS CLONING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TYMOVIRUS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Blawid, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Nagata, T.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1q8wEqKxx0QD65ZOfX60ve6WaKJzYOgYW/view?usp=drivesdk">https://drive.google.com/file/d/1q8wEqKxx0QD65ZOfX60ve6WaKJzYOgYW/view?usp=drivesdk</a>
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942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18945 25.06.2025 25.06.2025 Documentos solicitados