Construction of an agroinfectious clone of bean rugose mosaic virus using Gibson Assembly (Record no. 53089)

MARC details
000 -LEADER
fixed length control field 01945nam a2200289Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162422.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18946
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Construction of an agroinfectious clone of bean rugose mosaic virus using Gibson Assembly
490 0# - SERIES STATEMENT
Volume/sequential designation Virus genes, 53(3), p.495-499, 2017
520 3# - SUMMARY, ETC.
Summary, etc. Construction of agroinfectious viral clones usually requires many steps of cloning and sub-cloning and also a binary vector, which makes the process laborious, time-consuming, and frequently susceptible to some degree of plasmid instability. Nowadays, novel methods have been applied to the assembly of infectious viral clones, and here we have applied isothermal, single-step Gibson Assembly (GA)to construct an agroinfectious clone of Bean rugose mosaic virus (BRMV)using a small binary vector. The procedure has drastically reduced the cloning steps, and BRMV could be recovered from agroinfiltrated common bean twenty days after inoculation, indicating that the infectious clone could spread in the plant tissues and efficiently generate a systemic infection. The virus was also recovered from leaves of common bean and soybean cultivars mechanically inoculated with infectious clone two weeks after inoculation, confirming the efficiency of GA cloning procedure to produce the first BRMV agroinfectious clone to bean and soybean.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BEAN RUGOSE MOSAIC VIRUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element COMOVIRUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GIBSON ASSEMBLY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element INFECTIOUS CLONE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ISOTHERMAL REACTION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bijora, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Blawid, R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Costa, D. K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Aragão, F. J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Souto, E. R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Nagata, T.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1ascGZx8yi-hXE3oZe0haLwnyZEmwdYCD/view?usp=drivesdk">https://drive.google.com/file/d/1ascGZx8yi-hXE3oZe0haLwnyZEmwdYCD/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18946 25.06.2025 25.06.2025 Documentos solicitados