Distribution of sapogenins in morphological Medicago sativa L. parts: Comparison of various extraction techniques (Record no. 53092)

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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162422.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18949
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245 10 - TITLE STATEMENT
Title Distribution of sapogenins in morphological Medicago sativa L. parts: Comparison of various extraction techniques
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Separation Science, 43(3), p.671-680, 2020
520 3# - SUMMARY, ETC.
Summary, etc. Saponins in plant extracts were indirectly determined by estimation of the content of sapogenins. The first step of determination is extraction with high efficiency. One conventional extraction technique (maceration)and two modern ones (accelerated solvent extraction and supercritical fluid extraction)were compared. Methanol and ethanol were used as solvents or co-solvents. The results were supported by statistical analysis. Saponins were extracted from leaves, roots, and sprouts of Medicago sativa. Acid hydrolysis, purification, and determination by high-performance liquid chromatography with evaporative light scattering detector were used. The content of sapogenins was the highest in the roots. Smaller amounts of sapogenins were found in sprouts and the smallest ones in leaves. The main ingredient was medicagenic acid with mean concentration of 621.8 µg/g in roots, 456.7 µg/g in sprouts, and 471.3 µg/g in leaf extract. The highest content of sapogenins in extract was obtained after maceration with methanol; however, this method is nonselective in relation to biologically active compounds. Due to the possibility of using the obtained extracts with sapogenins in the cosmetic or pharmaceutical industry, the selection of extraction techniques and solvents is a very important aspect. Additionally, the chosen technique should be considered eco-friendly and consistent with the assumptions of "green chemistry."
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MEDIGACO SATIVA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element EXTRACTION METHODS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LIQUID CHROMATOGRAPHY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SAPOGENINS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element STATISTICAL ANALYSIS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kielbasa, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Krakowska-Sieprawska, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kowalkowski, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rafinska, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Buszewski, B.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1WmvW8KxF8xCyhHImmG89z4VZA72e-mnu/view?usp=drivesdk">https://drive.google.com/file/d/1WmvW8KxF8xCyhHImmG89z4VZA72e-mnu/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18949 25.06.2025 25.06.2025 Documentos solicitados