In Vitro Translation of Plant Viral RNA (Record no. 53140)

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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162423.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-18998
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245 10 - TITLE STATEMENT
Title In Vitro Translation of Plant Viral RNA
490 0# - SERIES STATEMENT
Volume/sequential designation Current Protocols in MicroBiology, Chapter 16:16K.1.1-16K.1.13., p.1-13, 2006
520 3# - SUMMARY, ETC.
Summary, etc. This unit describes the preparation of a wheat germ extract that provides all the soluble components of the plant translational machinery. Many RNA plant viruses have positive-strand genomes and the viral RNA serves as messenger RNA (mRNA). The preparation of mRNA by in vitro transcription is also described. The translation assay requires optimization of the amount of wheat germ extract, level of mRNA, and the concentration of Mg(2+)and K(+)for each mRNA. The translational efficiency of RNAs or mutants may be compared (e.g., capped versus uncapped RNAs to measure cap-independent translation)or the amount/size of the protein product may be determined.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Browning, K.S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mayberry, L.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1DK8cB2cQ58RB7b7_woXLbaeHC90o9o1z/view?usp=drivesdk">https://drive.google.com/file/d/1DK8cB2cQ58RB7b7_woXLbaeHC90o9o1z/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-18998 25.06.2025 25.06.2025 Documentos solicitados