Translation using a wheat-germ extract (Record no. 53141)
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| 000 -LEADER | |
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| fixed length control field | 02000nam a2200181Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162423.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-18999 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Translation using a wheat-germ extract |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | In Vitro Transcription and Translation Protocols. Series: Methods In Molecular Biology, Chapter 16, p.245-251, 1995 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Wheat-germ extract provides a reliable, convenient, and easy-to-use system to initiate translation from a wide variety of viral, prokaryotic, and eukaryotic mRNAs and produce full-length polypeptide products (1). Translation reactions in vitro may be directed by either mRNA isolated in vivo or by RNA templates transcribed in vitro. The mRNA synthesized in vitro should contain a 5? cap structure to enhance translational activity (2). Wheatgerm extract readily translates certain RNA preparations, such as those containing low concentrations of double-stranded RNA (dsRNA)or oxidized thiols, which am inhibitory to translation in reticulocyte lysate. Translation products may be further analyzed for processing events by the addition of canine pancreatic microsomal membranes (3). Steady improvements in the preparation of wheat-germ extract have allowed for performance comparable to that achieved in rabbit reticulocyte lysates (4). Studies of translational control are frequently done in wheat-germ extract because of the low level of endogenous mRNA present and lack of endogenous processing capabilities. Also, wheat-germ extract differs from rabbit reticulocyte lysate in that it does not contain endogenous transcription factors and, therefore, is the extract of choice for expressing eukaryotic transcription factors (5). |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Van Herwynen, J. F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Beckler, G. S. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1bOzgwwYfCyfzcPr6e_HdOCUby54na-ty/view?usp=drivesdk">https://drive.google.com/file/d/1bOzgwwYfCyfzcPr6e_HdOCUby54na-ty/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-18999 | 25.06.2025 | 25.06.2025 | Documentos solicitados |