MARC details
| 000 -LEADER |
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| fixed length control field |
02069nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162423.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-19001 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
In Vitro Translation Using Rabbit Reticulocyte Lysate |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
In vitro transcription and translation Protocols. Methods in Molecular Biology book series, 37. Chapter 14, p.215-232, 1995 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
The rabbit reticulocyte in vitro translation system, originally described by Pelham and Jackson (1), is widely used to identify mRNA species and characterize their products. Extensive descriptive protocols for the preparation of rabbit reticulocyte lysates have been previously described (2-4 and vol. 2 of this series). For the analysis of mRNA gene products, it is desirable to remove the endogenous mRNA present in the reticulocyte lysate with either micrococcal nuclease or nuclease S7. This eliminates any background translation products. Several factors are added to rabbit reticulocyte lysate to optimize translation efficiency. These include hemin (to prevent phosphorylation of initiation factor eIF2-a), an energy-generating system (e.g., phosphocreatine kinase and phosphocreatine), and calf liver tRNAs (to expand the range of mRNAs that can be translated). Other factors such as spermidine, 3?,5?-CAMP, GTP, and glucose-6-phosphate, can also stimulate translation, Protease inhibitors can be added to help prevent degradation of the translation product. Mg2+ and K+ salts are added to the lysate at levels suitable for translation of most RNA transcripts. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TRANSLATION PRODUCT |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MICROSOMAL MEMBRANE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
LABEL AMINO ACID |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RABBIT RETICULOCYTE LYSATE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MICROCOCCAL NUCLEASE |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Beckler, G. S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Thompson, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Oosbree, T. V. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1YM177O3sOkVnE_OQoKPV0QzgNi2b4_D1/view?usp=drivesdk">https://drive.google.com/file/d/1YM177O3sOkVnE_OQoKPV0QzgNi2b4_D1/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
