Successful polyploidy induction and detection in blackberry species by using an in vitro protocol (Record no. 53344)

MARC details
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fixed length control field 02632nam a2200277Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162426.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-19206
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245 10 - TITLE STATEMENT
Title Successful polyploidy induction and detection in blackberry species by using an in vitro protocol
490 0# - SERIES STATEMENT
Volume/sequential designation Scientia Horticulturae, 295, p.110850, 2022
520 3# - SUMMARY, ETC.
Summary, etc. Blackberries are widely used as a fresh fruit, while being highlighted as an anti-inflammatory and antioxidant source. Polyploidization is considered as a valuable and fast breeding method to develop new cultivars with enhanced characteristics. In this research, a procedure was adapted in vitro to generate polyploid plants (3x, 4x, 6x and 8x)in seven genotypes assigned to five blackberry species, including Rubus sanctus (3 genotypes), R. hirtus, R. caecious, R. persicus and R. discolor. Nodal segments were cultured in Murashige and Skoog media supplemented with oryzalin (0, 125, 250 and 375 mgL?1)or colchicine (0, 5, 10 and 15 mgL?1)for different durations (24, 48, 72 and 96 h). Results of cytological analysis showed no significant differences between the ultimate effects of the two anti-mitotic agents (i.e. colchicine and oryzalin), whereas each species reacted differently to polyploidy induction. The best result was obtained in the R. sanctus genotype F (Sari), using 10 mgL?1 oryzalin for 48 h. Manipulative treatments led to the release of octaploid blackberry for the first time. A morphometric analysis of plant leaves showed that polyploidy affected the guard cells, increased the cell size and reduced cell count. The greenness, petiole length and chlorophyll content of leaves were significantly higher in the hexaploid R. sanctus genotype C (Lahijan), while the thickness and size of leaves were significantly larger and the spine length was significantly longer in the octaploid R. persicus. The developed in vitro procedure for polyploidy induction and detection is feasible and could be used as a promising tool to create blackberry cultivars with enhanced fruit characteristics, along with possible resistance to environmental stress.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RUBUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element COLCHICINE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ORYZALIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FLOW CYTOMETRY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MORPHOLOGY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHROMOSOME COUNTING
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sabooni, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gharaghani, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Jowkar, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Eshghi, S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1Ab5-RZVkVTcQqkHG9Y4LurNok6za5Gxj/view?usp=drivesdk">https://drive.google.com/file/d/1Ab5-RZVkVTcQqkHG9Y4LurNok6za5Gxj/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-19206 25.06.2025 25.06.2025 Documentos solicitados