Synthetic promoters capable of driving robust nuclear gene expression in the green alga Chlamydomonas reinhardtii (Record no. 53359)
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| fixed length control field | 02459nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162427.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-19221 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Synthetic promoters capable of driving robust nuclear gene expression in the green alga Chlamydomonas reinhardtii |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Algal Research, 15, p.135-142, 2016 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Algae have enormous potential as bio-factories for the efficient production of a wide array of high-value products, and eventually as a source of renewable biofuels. However, tools for engineering the nuclear genomes of algae remain scarce and limited in functionality. In this study, synthetic algal promoters (saps)were generated as a tool for increasing nuclear gene expression and as a model for understanding promoter elements and structure in green algae. Promoters were generated to mimic native cis-motif elements, structure, and overall nucleotide composition of top expressing genes from Chlamydomonas reinhardtii. Twenty five saps were used to drive expression of a fluorescent reporter in transgenic algae. A majority of the promoters were functional in vivo and seven were identified to drive expression of the fluorescent reporter better than the current best endogenous promoter in C. reinhardtii, the chimeric hsp70/rbs2 promoter. Further analysis of the best synthetic promoter, sap11, revealed a new DNA motif essential for promoter function that is widespread and highly conserved in C. reinhardtii. These data demonstrate the utility of synthetic promoters to drive gene expression in green algae, and lays the groundwork for the development of a suite of saps capable of driving the robust and complex gene expression that will be required for algae to reach their potential as an industrial platform for photosynthetic bio-manufacturing. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ALGAL ENGINEERING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | C. REINHARDTII |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SYNTHETIC BIOLOGY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | NUCLEAR PROMOTERS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CIS-MOTIFS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | FLOW CYTOMETRY |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Scranton, M. A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ostrand, J. T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Georgianna, D. R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lofgren, S. M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Li, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ellis, R. C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mayfield, S. P. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1p2BLb_V73RTFvIzPCB-kG5VLAIcwZC-T/view?usp=drivesdk">https://drive.google.com/file/d/1p2BLb_V73RTFvIzPCB-kG5VLAIcwZC-T/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-19221 | 25.06.2025 | 25.06.2025 | Documentos solicitados |