In vitro desiccation tolerance of the epiphytic Ghost Orchid, Dendrophylax lindenii (Lindl.)Benth x. Rolfe (Record no. 53366)

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control field 20250625162427.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-19228
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245 10 - TITLE STATEMENT
Title In vitro desiccation tolerance of the epiphytic Ghost Orchid, Dendrophylax lindenii (Lindl.)Benth x. Rolfe
490 0# - SERIES STATEMENT
Volume/sequential designation In Vitro Cellular & Developmental Biology-Plant, 55(1), p.60-70, 2019
520 3# - SUMMARY, ETC.
Summary, etc. The Ghost Orchid, Dendrophylax lindenii (Lindl.)Benth x. Rolfe, is a rare and endangered epiphytic orchid native to south Florida and Cuba. The orchid is considered difficult to propagate under greenhouse conditions, requiring high humidity and low air movement. In contrast, the orchid's native habitat seasonally dries out with decreased precipitation and humidity. This suggests some level of desiccation tolerance. Ghost Orchid plants were assessed for potential desiccation tolerance and ability to recover from desiccation stress under in vitro conditions. In vitro-derived plants were placed into sterile baby food jars and transferred to chambers maintained at 10 percent relative humidity, which is extremely low compared to relative humidity levels (40-100 percent ) recorded under natural field conditions. Plants were removed every week for 4 wk and recovered on P723 medium supplemented with banana powder for 4 wk. Data were collected at the initiation of the experiment, after the desiccation periods, and after 4 wk in vitro recovery. Ghost Orchid plants demonstrated extremely high desiccation tolerance. Even after 4 wk desiccation, plant survival was observed at 79.2 percent after recovery. Desiccated plants exhibited significant decreases in tissue water potential (??18.44 MPa), fresh weight (65.5 percent loss), and water content (14.2 percent ); however, high plant survival was still observed under these conditions similar to poikilohydric plants. Overall, the Ghost Orchid demonstrated high desiccation tolerance, which should be considered for future greenhouse culture and for its application in the direct field establishment of in vitro-derived plants without greenhouse acclimatization.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DESICCATION TOLERANCE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GHOSTORCHID
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element IN VITRO
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RELATIVE HUMIDITY
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Coopman, J. C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kane, M. E.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1MEyJwY3RVAja80QiRVmbrjb_y_XUAuLW/view?usp=drivesdk">https://drive.google.com/file/d/1MEyJwY3RVAja80QiRVmbrjb_y_XUAuLW/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-19228 25.06.2025 25.06.2025 Documentos solicitados