Isolation and characterisation of nematicidal compound, leolorin C, from Leonotis leonurus acetone leaf extract (Record no. 53371)
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| 000 -LEADER | |
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| fixed length control field | 02817nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162427.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-19233 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Isolation and characterisation of nematicidal compound, leolorin C, from Leonotis leonurus acetone leaf extract |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of EthnoPharmacology, 284, p.114802, 2022 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Ethnopharmacological relevance: Leonotis leonurus (L.)R.Br. (Lamiaceae)is a perennial shrub native to South Africa used to treat various diseases including digestive tract problems, intestinal worms and constipation. Aim of the study: The aim was to isolate and characterise nematicidal compounds from leaves of L. leonurus. Materials and methods: Bioassay-guided fractionation was carried out using the free-living nematode Caenorhabditis elegans as a model organism. Structural elucidation of the purified compound was carried out using NMR spectroscopic analyses and UPLC-QTOF-MS. The fractions and the isolated compound were tested for nematicidal activity on motility of plant-parasitic Meloidogyne incognita juveniles (J2s)and J2 hatch inhibition. Further screening was done to determine the minimum inhibitory concentration (MIC)of the fractions against bacterial phytopathogens and cytotoxicity against Vero kidney cells. Results: Leoleorin C isolated from L. leonurus had moderate activity against C. elegans juveniles (34 percent ) but was not active against J2 motility and J2 hatch of M. incognita. Thus, activity against the free-living C. elegans did not correspond with efficacy against plant-parasitic nematodes. Leoleorin C was not active against the tested bacterial phytopathogens, but some activity was observed in the bioautography assay against Clavibacter michiganensis subsp. michiganensis, the organism causing bacterial canker in tomatoes. The plant extract, fractions and leolorin C were relatively non-toxic to Vero cells with LC50 values greater than 0.01 mg/mL. Conclusion: The crude extract of L. leonurus and fractions may be useful in developing complementary treatments for controlling nematodes and phytopathogens. This study does not support the use of free-living nematodes as a model to isolate anti-parasitic compounds from plants. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | LEONOTIS LEONURUS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ANTHELMINTIC |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ANTIMICROBIAL |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CYTOTOXICITY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | LEOLEORIN C |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CAENORHABDITIS ELEGANS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Makhubu, F. N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nkadimeng, S. M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Fouche, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Khosa, M. C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mcgaw, L. J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1t7qt5JuB8MQq1KBiPRV8n_JI4CXhcg7E/view?usp=drivesdk">https://drive.google.com/file/d/1t7qt5JuB8MQq1KBiPRV8n_JI4CXhcg7E/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-19233 | 25.06.2025 | 25.06.2025 | Documentos solicitados |