MARC details
| 000 -LEADER |
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| fixed length control field |
02708nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162428.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-19328 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Melatonin different increasing the cryopreservation recovery rate of shoot tips of Chrysanthemum morifolium cv. Chuju by regulating the level of oxidative stress |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Plant Cell, Tissue and Organ Culture , 149, p.785-797, 2022 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Cryopreservation is vital to the preservation of genotypic diversity of plant species. In the present study, an efficient procedure for cryopreservation of Chrysanthemum cv. Chuju shoot tips was established for the first time. The adventitious shoot clusters of Chuju were transferred to MS medium fortified with 100 µM melatonin (MLT)and cultured at 4 °C for 4 days. Then, the shoot tips were excised and precultured on MS medium supplemented with 0.4 M sucrose for 4 days at 4 °C in the dark. After that, the shoot tips were loaded in 60 percent plant vitrification solution 2 (PVS2)for 20 min at 0 °C, followed by immersion in 100 percent PVS2 solution for 60 min. Then, put the shoot tips into liquid nitrogen (LN,???196 °C). After at least 1 h, the LN-stored shoot tips were rapidly rearmed for 2 min at 39 °C, and liquid MS medium containing 1.2 M sucrose was used as the unloading solution for 15 min. Finally, the cells were postcultured in recovery medium for shoot regeneration. Under these conditions, 65.7 percent of cryopreserved shoot tips survived and regenerated. Further biochemical and histological analysis showed that exogenous MLT could improve the survival rate by regulating the level of oxidative stress and maintaining the morphological structure of shoot tip cells, which provided a new method for exploring the mechanism of MLT in cryopreservation. Intersimple sequence repeat analysis did not reveal any polymorphic bands in regenerants recovered from the shoot tips of Chuju. We expect that our results will facilitate the successful application of MLT in the cryopreservation of rare and commercially important plant germplasm. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
BIOCHEMICAL ANALYSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CRYOPRESERVATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GENETIC FIDELITY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HISTOLOGICAL ANALYSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MELATONIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MORPHOLOGICAL STRUCTURE |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Su, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wang, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Kan, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Yao, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Ding, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Chen, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wu, L. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1IRH2xr4Pkegb8DtRUzcCapPGhv4Ogoza/view?usp=drivesdk">https://drive.google.com/file/d/1IRH2xr4Pkegb8DtRUzcCapPGhv4Ogoza/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
