Detection of RNA-protein interactions using a highly sensitive non-radioactive electrophoretic mobility shift assay (Record no. 53485)

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fixed length control field 01872nam a2200289Ia 4500
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control field MX-MdCICY
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control field 20250625162429.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-19351
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Title Detection of RNA-protein interactions using a highly sensitive non-radioactive electrophoretic mobility shift assay
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Volume/sequential designation Electrophoresis, 40(9), p.1365-1371, 2019
520 3# - SUMMARY, ETC.
Summary, etc. Electrophoretic mobility shift assay (EMSA)is a sensitive technique useful in the identification and characterization of protein interactors with nucleic acids. This assay provides an efficient method to study DNA or RNA binding proteins and to identify nucleic acid substrates. The specific interaction plays important roles in many biological processes such as transcription, translation, splicing, and global gene expression. In this article, we have modified the EMSA technique and developed a non-radioactive straightforward method to study and determine RNA-protein interactions. The labeling of target RNAs by 3'-end biotinylation and the detection of biotin reactivity to streptavidin-conjugated horseradish peroxidase is a highly sensitive approach capable to detect the formation of RNA-protein complexes. Overall, we provide a complete technical guide useful to determine in vitro RNA-protein interactions and analyze RNA target specificity.
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Topical term or geographic name entry element EMSA
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Topical term or geographic name entry element GEL RETARDATION
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Topical term or geographic name entry element GEL SHIFT
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Topical term or geographic name entry element RNA
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Topical term or geographic name entry element RNA-PROTEIN INTERACTIONS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Daras, G.
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Personal name Alatzas, A.
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Personal name Tsitsekian, D.
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Personal name Templalexis, D.
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Personal name Rigas, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hatzopoulos, P.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/17MSXFhLnQ_lQzP3MuHCBhW_nzF-e18kC/view?usp=drivesdk">https://drive.google.com/file/d/17MSXFhLnQ_lQzP3MuHCBhW_nzF-e18kC/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-19351 25.06.2025 25.06.2025 Documentos solicitados