Culture of immature embryos of coconut, Cocos nucifera L: Callus proliferation and somatic embryogenesis (Record no. 53500)

MARC details
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control field MX-MdCICY
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control field 20250625162429.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-19369
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245 10 - TITLE STATEMENT
Title Culture of immature embryos of coconut, Cocos nucifera L: Callus proliferation and somatic embryogenesis
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Science, 62(2), p.247-253, 1989
520 3# - SUMMARY, ETC.
Summary, etc. The culture of immature embryos of coconut (Cocos nucifera L var. typica)was attempted with a view to developing a technique for clonal propagation. Embryos, 6-7 months postanthesis, produced whitish, compact, embryogenic callus tissues in the presence of 12-20 ?M 2,4-D. Callus was maintained by subculture on a medium of the same composition. About 50 percent of the callus cultures produced globular embryos when transferred to 8 ?M 2,4-D. Embryos in 22 percent of these cultures germinated and produced shoots, 6 mm long, when 6-benzylamino purine and kinetin (10 ?M each)were incorporated into the culture medium. The age of the embryo was an important factor determining callus proliferation and subsequent embryogenesis. More than 50 percent of embryos excised from 6-7-month-old nuts produced embryogenic callus tissues. Five to six-month-old nuts were not mature enough for existing the embryos. Embryos from older nuts (8 months and above)germinated in culture. Only a small portion of the immature embryo produced embryogenic callus tissues. The furure root region proliferated into a brownish callus which in turn produced roots profusely. The cotyledon of the immature embryo expanded and developed into a haustorium without any sign of callus formation. The shoot apex produced a few plumular leaves but no callusing was observed.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element COCOS NUCIFERA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element IMMATURE EMBRYO
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC EMBRYOGENESIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element EMBRYOGENIC CALLUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element COTYLEDON
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element POSTANTHESIS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Karunaratne, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Periyapperuma, K.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1W9d1JhHxdJ_P1NX_HQV5cWFK68pyJq_o/view?usp=drivesdk">https://drive.google.com/file/d/1W9d1JhHxdJ_P1NX_HQV5cWFK68pyJq_o/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-19369 25.06.2025 25.06.2025 Documentos solicitados