Transient reduction in secreted 32 kD chitinase prevents somatic embryogenesis in the carrot (Daucus carota L.)variant ts11 (Record no. 53501)

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control field 20250625162429.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-19370
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245 10 - TITLE STATEMENT
Title Transient reduction in secreted 32 kD chitinase prevents somatic embryogenesis in the carrot (Daucus carota L.)variant ts11
490 0# - SERIES STATEMENT
Volume/sequential designation Developmental genetics, 16(4), p.332-343, 1995
520 3# - SUMMARY, ETC.
Summary, etc. At the nonpermissive temperature, somatic embryos of the temperature-sensitive (ts)carrot (Daucus carota L.)cell variant ts11 only proceed beyond the globular embryo stage in the presence of medium conditioned by wild-type cells. The causative component in the conditioned medium has been identified as an acidic 32 kD endochitinase. An antiserum raised against the 32 kD chitinase detected this protein in culture medium from ts11 embryo cultures grown at the permissive temperature as well as at the nonpermissive temperature. No difference in biochemical characteristics or in effect on ts11 embryo development could be detected between the 32 kD chitinase purified from wild-type cultures and the chitinase from ts11 cultures grown at the permissive or at the nonpermissive temperature. Compared to the amount present in a ts11 embryo culture at the permissive temperature, a reduction in the amount of 32 kD chitinase was observed during the temperature-sensitive period at the nonpermissive temperature. These results imply that the arrested embryo phenotype of ts11 is not the result of a structural difference in its 32 kD chitinase, but is the result of a transient decrease in the amount of 32 kD chitinase present. Morphological observations indicate that the ts11 phenotype is pleiotropic and also affects the cell wall of nonembryogenic cells.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC EMBRYOGENESIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TEMPERATURE-SENSITIVE MUTANT TS 7 I
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHITINASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CARROT (DAUCUS CAROTA L.)
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name De Jong, A. J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hendriks, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Meijer, E. A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Penning, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Schiavo, F. L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Terzi, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name De Vries, S. C.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1fud9YKqZLF7e9lv1nZHfmWMdmv50QsiC/view?usp=drivesdk">https://drive.google.com/file/d/1fud9YKqZLF7e9lv1nZHfmWMdmv50QsiC/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-19370 25.06.2025 25.06.2025 Documentos solicitados