MARC details
| 000 -LEADER |
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| fixed length control field |
02334nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162431.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-19459 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
miR156 regulates somatic embryogenesis by modulating starch accumulation in citrus |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Journal of Experimental Botany, https://doi.org/10.1093/jxb/erac248, 2022 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Somatic embryogenesis (SE)is a major regeneration approach for in vitro cultured tissues of plants, including citrus. However, SE capability is difficult to maintain, and recalcitrance to SE has become a major obstacle to plant biotechnology. We previously reported that miR156-SPL modules regulate SE in citrus callus. However, the downstream regulatory pathway of the miR156-SPL module in SE remains unclear. In this study, we found that the transcription factors (TFs)CsAGL15 and CsFUS3 bind to the CsMIR156A promoter and activate its expression. Suppression of csi-miR156a function leads to up-regulation of four target genes CsSPLs and reduction of SE efficiency. In the STTM-miR156a overexpressed callus (MIM156), the number of amyloplasts and starch content were significantly reduced, and genes involved in starch synthesis and transport were down-regulated; csi-miR172d was down-regulated, whereas the target genes CsTOE1.1 and CsTOE1.2, which inhibit the expression of starch biosynthesis genes, were up-regulated. In our working model, CsAGL15 and CsFUS3 activate csi-miR156a, which represses CsSPLs and further regulates csi-miR172d and CsTOEs, thus to alter starch accumulation level in callus cells and regulate SE in citrus. This study elucidates the pathway of miR156-SPLs and miR172-TOEs mediated regulation of SE, and provides clues to enhancing SE capability in citrus. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CALLUS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CITRUS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MIR156 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MIR172 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SOMATIC EMBRYOGENESIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
STARCH |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Feng, M. Q. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Lu, M. D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Long, J. M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Yin, Z. P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Jiang, N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wang, P. B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wu, X. M. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/15iuq3Xsgdk6bKK37yvs-V_om0uwm8TMj/view?usp=drivesdk">https://drive.google.com/file/d/15iuq3Xsgdk6bKK37yvs-V_om0uwm8TMj/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
