MARC details
| 000 -LEADER |
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| fixed length control field |
02599nam a2200301Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162431.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-19461 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Tetraploid induction through somatic embryogenesis in Panax vietnamensis Ha et Grushv. by colchicine treatment |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Scientia Horticulturae, 303, p.111254, 2022 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
In the present study, the protocol of polyploid induction via somatic embryogenesis of the Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.)(2n = 2x = 24)was developed. Ex vitro leaf explants were disinfected with AgNPs at concentrations of 0.05, 0.1, 0.2 and 0.5 g/L for 5, 10, 15, 20 and 30 min and cultured on MS medium supplemented with 1.0 mg/L 2,4-D, 0.5 mg/L NAA and 0.2 mg/L Kin for somatic embryogenesis. Globular embryos induced from the leaf explants were treated with colchicine by immersed method at different concentrations of 0, 0.1, 0.2, 0.3, 0.5 and 0.7 percent for 24, 36, 48 and 72 h. Then, these embryos were cultured on medium supplemented with 1.0 mg/L 2,4-D, 0.5 mg/L NAA and 0.2 mg/L Kin for secondary embryos induction. The ploidy level was identified by counting chromosomes of the root tip of plantlets derived from these secondary embryos. The results showed that the survival rate and somatic embryo induction the highest (65.56 percent and 29.89 percent, respectively)at 0.2 g/L AgNPs for 20 min. For colchicine treatment, the increase in the concentration and exposure time of colchicine decreased the survival rate of explants and the number of secondary embryos but increased the abnormal secondary embryo formation. Identifying the ploidy level showed that the treatments with colchicine concentrations from 0.3 percent to 0.5 percent for 48 h lead to tetraploid induction rates from 22.22 percent to 25.92 percent. The results also revealed that the tetraploids (2n = 4x = 48)had larger stomatal size, lower stomatal density, denser stomatal chloroplast density, and better growth than did the diploids. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ABNORMAL EMBRYOS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
COLCHICINE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
NGOC LINH GINSENG |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
STOMATA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TETRAPLOID INDUCTION |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Phong, T. H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Tung, H. T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Khai, H. D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Anh, T. T. L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Mai, N. T. N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Luan, V. Q. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Nhut, D. T. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1YJvVYRhMlWK9stLJLyyI6WFDeKiIAZ6Y/view?usp=drivesdk">https://drive.google.com/file/d/1YJvVYRhMlWK9stLJLyyI6WFDeKiIAZ6Y/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
