MARC details
| 000 -LEADER |
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| fixed length control field |
03500nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162431.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-19472 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
In vitro large-scale propagation and genetic fidelity of Toona sinensis (Juss.)M. Roem., an economically important vegetable tree |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Plant Cell, Tissue and Organ Culture , https://doi.org/10.1007/s11240-022-02351-z, 2022 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Toona sinensis (Juss.)M.Roem., is an economically important seasonable vegetable tree with a wide range of nutritional, medicinal, and industrial values. The existing breeding methods can no longer sustainably meet the growing and annual production demands for T. sinensis buds. An efficient and cost-effective in vitro large-scale micropropagation procedure for T. sinensis was developed from shoot stems with axillary or terminal buds through the processes of callus elimination, multiple shoot induction and hydroponic acclimation of the regenerated plantlets. Plant growth regulators, explant type, explant age and activated charcoal (AC)play an important role in multiple shoot induction and callus reduction. The highest shoot induction frequency (97.8 percent)with a mean number of shoots equal to 7.6 and a relatively smaller callus (0.3 cm in diameter)was obtained from the in vitro culture of 75-day-old shoot stems with axillary buds in Murashige and Skoog (MS)medium augmented with 1.0 mg l?¹ N6-benzyladenine (6-BA)and 0.2 mg l?¹ indole-3-acetic acid (IAA). In addition, a complete plantlet with strong roots and mature leaves could be obtained directly by culturing the shoot stem segment on medium containing at least 0.5 g l?¹ AC, which reveals a new direction for the industrialized propagation of T. sinensis. Among the PGRs tested, a low concentration (0.5 mg l?¹)of zeatin (ZT)was found to be the most conducive to shoot elongation. We also found that low concentrations of auxin, activated carbon, sucrose and a low macronutrient strength are more beneficial for in vitro adventitious root formation. The highest rooting response (100 percent)with 5.7 roots produced per shoot was achieved in 1/4 MS medium with the addition of 0.5 mg l?¹ potassium salt of indole butyric acid (K-IBA), 0.5 g l?¹ AC, and 10.0 g l?¹ sucrose. In addition, the major challenge of the low transplanting survival rate of regenerated T. sinensis plants was solved by adopting an ex-vitro hydroponic domestication strategy in a mini-incubator. The plants were successfully hardened in a transplanting mixture of garden soil, perlite, vermiculite and Pindstrup substrate (3:1:1:3), and the transplanting survival rate was 95.3 percent. Inter simple sequence repeat (ISSR)assessment revealed no difference between the regenerated plants and the mother plants. The micropropagation protocol established in this study will lay the foundation for germplasm conservation of T. sinensis and its sustainable utilization in food. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TOONA SINENSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MULTIPLE SHOOT INDUCTION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CALLUS REDUCTION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HYDROPONIC DOMESTICATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GENETIC STABILITY ASSESSMENT |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Hou, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wang, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Su, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Ding, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wu, L. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1WBv010pLPtBZafFqC2vo2sVPqJOKbttM/view?usp=drivesdk">https://drive.google.com/file/d/1WBv010pLPtBZafFqC2vo2sVPqJOKbttM/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
