Development of polyclonal antibodies-based serological methods and a DIG-labelled DNA probe-based molecular method for detection of the Vicia cryptic virus-M in field plants (Record no. 53601)

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fixed length control field 02252nam a2200265Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162431.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-19475
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245 10 - TITLE STATEMENT
Title Development of polyclonal antibodies-based serological methods and a DIG-labelled DNA probe-based molecular method for detection of the Vicia cryptic virus-M in field plants
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Virological Methods, 299, p.114331, 2022
520 3# - SUMMARY, ETC.
Summary, etc. Vicia cryptic virus M (VCV-M), a member of the genus Amalgavirus of the family Amalgaviridae, was first identified in 2009 in a Vicia faba Linn. planting in Hangzhou, Zhejiang Province, China. However, there has been no further research on the biological features of VCV-M to date and the viral particles and coat protein (CP)have not been identified. The putative CP of VCV-M was predicted from the viral genomic RNA. In this study, a recombinant version of the putative CP of VCV-M (His-CPVCV-M)was produced and used to prepare a polyclonal antiserum against the His-CPVCV-M. Using this antiserum, a Western blot, an immuno-dot-blot and an enzyme-linked immunosorbent assay were developed for testing field samples of V. faba for the presence of VCV-M. Additionally, a digoxigenin (DIG)-labelled DNA probe-based Northern blot assay was established for VCV-M genome detection in field samples. The results showed that both the serological and nucleic acid assays could accurately and sensitively detect VCV-M in V. faba. This research represented the first confirmed expression of the putative CP of VCV-M in infected V. faba tissues. The serological and nucleic acid assays provided two complementary methods for VCV-M detection which could contribute to seed quality control and production increases of V. faba crops.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DIG-LABELLED PROBE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element POLYCLONAL ANTISERUM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element VICIA CRYPTIC VIRUS M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhang, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhuang, X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Xu, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gan, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name He, Z.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chen, J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1bRfBtZf93PIM6N0rEmC3PPq1-3cH5d4f/view?usp=drivesdk">https://drive.google.com/file/d/1bRfBtZf93PIM6N0rEmC3PPq1-3cH5d4f/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-19475 25.06.2025 25.06.2025 Documentos solicitados