A modified cotton-wool column method for the rapid recovery of DNA from agarose gel slice (Record no. 53694)
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| fixed length control field | 02068nam a2200205Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162433.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-19570 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | A modified cotton-wool column method for the rapid recovery of DNA from agarose gel slice |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of Rapid Methods & Automation in MicroBiology, 16(1), p.55-61, 2008 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | A previous report has described a simple and inexpensive cotton-wool column method for the recovery of DNA from agarose gel slice. However, it takes 2 h from start to finish. Here we present a simplified version of the technique which can cut hands-on time to within 20 min while retaining a fair DNA yield. Agarose gel slice was mashed before conducting centrifugation of the cotton-wool column tube, which enhanced DNA yield by at least 50 percent. Only one spin was required with a 30-35 percent DNA recovery rate, a second spin after treatment of agarose sediment in the tube with microwave would add another several percent. DNA obtained can be efficiently amplified by polymerase chain reaction (PCR)or subcloned. This provides a simple, rapid and cheap alternative to commercial kits. The mashed agarose gel slice alone or with water is used for centrifugation with the best efficiency. The flowthrough can be directly used for polymerase chain reaction (PCR)amplifications, which would only take less than 10 min to obtain. The flowthrough must be ethanol precipitated if other molecular manipulations such as cloning are to be conducted, which will require another 10 min. One sample costs around 1 US cent to process with the cotton-wool column method in developing countries. It will be a rapid and reliable alternative to expensive commercial kits. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Liu, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Xu, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Luo, Z. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Liu, Q. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1m1KSruY8YHEQ-DpVnz2RlmmGTAEivznA/view?usp=drivesdk">https://drive.google.com/file/d/1m1KSruY8YHEQ-DpVnz2RlmmGTAEivznA/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-19570 | 25.06.2025 | 25.06.2025 | Documentos solicitados |