Simple and inexpensive long-term preservation methods for Phytophthora infestans (Record no. 53721)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02466nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162433.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-19598 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Simple and inexpensive long-term preservation methods for Phytophthora infestans |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of microBiological methods, 152, p.80-85, 2018 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Phytophthora infestans is one of the most notorious pathogen among Phytophthora species causing potato late blight disease. Stable and long-term preservation of this pathogen is essential for biological research and fungicide screening. The aim of this study was to find a suitable long-term preservation method for P. infestans. We adjusted the storage temperature, made a slight modification to the rye seed method, and compared the influence of four preservation methods (the mineral oil method, the sterile water method, the rye seed method, and the modified rye seed method)on survival, growth and virulence of four isolates of P. infestans. The results showed that all four methods maintained high viability of the tested P. infestans isolates, but the two rye seed methods were the best ways to maintain 100 percent viability of the P. infestans isolates without contamination. The four preservation methods did not significantly influence growth or morphological characteristics of the P. infestans isolates. The impacts of the four methods on the virulence of the four P. infestans isolates were isolate-specific. For isolates YF3 and 64093, all four methods were suitable for maintaining their virulence. Whilst for isolate HQK8-3, the rye seed and sterile water methods were more suitable to maintain its virulence than the other two methods. For isolate 32835, storage under mineral oil was the best method for maintaining its virulence. In view of these results, it is recommended P. infestans should be stored by several different storage methods to ensure the safety and stability of the isolates. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PHYTOPHTHORA INFESTANS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PRESERVATION METHOD |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SURVIVAL |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | VIRULENCE |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cui, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ren, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yun, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hou, Q. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Feng, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Liu, H. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1ELxe-yr0Ouop_vsgpC6aP_hLp8OZzfG0/view?usp=drivesdk">https://drive.google.com/file/d/1ELxe-yr0Ouop_vsgpC6aP_hLp8OZzfG0/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-19598 | 25.06.2025 | 25.06.2025 | Documentos solicitados |