An efficient protocol for inoculation of Fusarium graminearum in detached leaves of Arabidopsis (Record no. 53798)

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fixed length control field 02285nam a2200289Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162435.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-19675
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245 10 - TITLE STATEMENT
Title An efficient protocol for inoculation of Fusarium graminearum in detached leaves of Arabidopsis
490 0# - SERIES STATEMENT
Volume/sequential designation Tropical Plant Pathology, 47(3), p.353-361, 2022
520 3# - SUMMARY, ETC.
Summary, etc. Fusarium graminearum is the main cause of Fusarium head blight, a fungal disease that reduces yield and affects the quality of wheat and other small grains. Genetic resistance is the ideal method for disease control; however, there are not yet cultivars with sufficient resistance levels to withstand an epidemic. Genetic engineering strategies such as gene silencing, host-induced gene silencing (HIGS), overexpression, and genome editing are promising, but many target genes need to be analyzed to find a suitable one. Arabidopsis thaliana is a model plant that is also susceptible to F. graminearum. Although this interaction was reported nearly two decades ago, consistent infection and symptoms are not always obtained. The availability of an efficient inoculation method is essential for studying plant-pathogen interaction. This work aimed at testing protocols for inoculating F. graminearum in detached leaves of A. thaliana which varied in inoculation site (leaf sides), wound (abrasion or hole injury), and inoculum type (mycelium agar disk or spore). We found that a mycelium agar disk placed on an abrasion injury at the adaxial leaf side was the most efficient (highest lesion size, incidence, and easiness to visualize the symptoms caused by the pathogen)method to inoculate F. graminearum in detached leaves of Arabidopsis.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FUSARIUM HEAD BLIGHT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element INOCULATION ASSAY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PROTOCOL
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ARABIDOPSIS THALIANA
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Roesler, E. A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Balbinott, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Schroeder, R. G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yamazaki-Lau, E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lima, M. I. P. M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Brammer, S. P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Deuner, C. C.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1v0vSJDcjoG1FLrOYKovEAWLTO6WbFZ6n/view?usp=drivesdk">https://drive.google.com/file/d/1v0vSJDcjoG1FLrOYKovEAWLTO6WbFZ6n/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-19675 25.06.2025 25.06.2025 Documentos solicitados