Extraction of hyaluronic acid (HA)from rooster comb and characterization using flow field-flow fractionation (FlFFF)coupled with multiangle light scattering (MALS) (Record no. 53954)

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control field 20250625162438.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-19838
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245 10 - TITLE STATEMENT
Title Extraction of hyaluronic acid (HA)from rooster comb and characterization using flow field-flow fractionation (FlFFF)coupled with multiangle light scattering (MALS)
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of separation Science, 33(22), p.3530-3536, 2010
520 3# - SUMMARY, ETC.
Summary, etc. Hyaluronic acid (HA)was extracted in a relatively large scale from rooster comb using a method similar to that reported previously. The extraction method was modified to simplify and to reduce time and cost in order to accommodate a large-scale extraction. Five hundred grams of frozen rooster combs yielded about 500 mg of dried HA. Extracted HA was characterized using asymmetrical flow field-flow fractionation (AsFlFFF)coupled online to a multiangle light scattering detector and a refractive index detector to determine the molecular size, molecular weight (MW)distribution, and molecular conformation of HA. For characterization of HA, AsFlFFF was operated by a simplified two-step procedure, instead of the conventional three-step procedure, where the first two steps (sample loading and focusing)were combined into one to avoid the adsorption of viscous HA onto the channel membrane. The simplified two-step AsFlFFF yielded reasonably good separations of HA molecules based on their MWs. The weight average MW (M(w)) and the average root-mean-square (RMS)radius of HA extracted from rooster comb were 1.20×10(6)and 94.7 nm, respectively. When the sample solution was filtered through a 0.45 ?m disposable syringe filter, they were reduced down to 3.8×10(5)and 50.1 nm, respectively.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ASYMMETRICAL FLOW FIELD-FLOW FRACTIONATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HYALURONIC ACID
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MULTIANGLE LIGHT SCATTERING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MOLECULAR WEIGHT DISTRIBUTION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ROOSTER COMB
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kang, D. Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kim, W. S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Heo, I. S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Park, Y. H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lee, S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1uZzyoyGRpZYFOEey6zqK5obZ6Z3q9B0p/view?usp=drivesdk">https://drive.google.com/file/d/1uZzyoyGRpZYFOEey6zqK5obZ6Z3q9B0p/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-19838 25.06.2025 25.06.2025 Documentos solicitados