MARC details
| 000 -LEADER |
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| fixed length control field |
02971nam a2200301Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162438.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-19844 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Quantification of six types of cytokinins: Integration of an ultra-performance liquid chromatographic-electrospray tandem mass spectrometric method with antibody based immunoaffinity columns equally recognizing cytokinins in free base and nucleoside forms |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Journal of Chromatography A, 1682, p.463497, 2022 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Cytokinins (CTKs)exist in various types in plants. The accurate quantification of free base and nucleoside types of cytokinins are helpful for better understanding their physiological role. In the present study, antibodies against trans-zeatin riboside (tZR)and N6-isopentenyladenine riboside (iPR)antibodies with equal recognition to free base and nucleoside cytokinins were developed. The cross-reactivity of tZR mAb 3G101G7 with tZR, trans-zeatin (tZ), dihydrozeatin riboside (DHZR), dihydrozeatin (DHZ), iPR, and N6-isopentenyladenine (iP)was 100.0 percent, 95.7 percent, 19.1 percent, 18.0 percent, 1.1 percent, and 0.7 percent, and that of iPR mAb 5C82F1 with above-mentioned 6 types of cytokinins was 1.5 percent, 1.4 percent, 5.7 percent, 3.1 percent, 100.0 percent and 92.6 percent, respectively. The obtained antibodies were used to prepare two immunoaffinity columns (IAC). The elution efficiencies of tZR 3G101G7-IAC for tZ and tZR, DHZ and DHZR and of iPR 5C82F1-IAC for iP and iPR were almost no difference with the same loading amount on their corresponding IACs. Subsequently, six types of cytokinins in mepiquat chloride (MC)-treated cotton (Gossypium hirsutum L.)roots were determined by IACs combined with ultra-performance liquid chromatography-electrospray tandem mass spectrometry (UPLC-ESI-MS/MS). The contents of tZR, iPR and DHZR were increased by 9.3?38.5 percent, 6.6?23.5 percent, and 30.1?110.0 percent, respectively, whereas those of tZ and iP were reduced by 5.3?20.0 percent and 27.7?32.1 percent, respectively. The decreased tZ and iP levels led to the ratio of auxin-to-active cytokinins increase to promote lateral root initiation in MC-treated cotton seeding. Integration of the IACs equally recognizing cytokinins in their free base and nucleoside forms with UPLC-ESI-MS/MS can accurately quantify different cytokinins in plant tissues. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CYTOKININS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GOSSYPIUM HIRSUTUM L |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
IMMUNOAFFINITY CLEANUP |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MONOCLONAL ANTIBODY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
UPLC-ESI-MS/MS |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zhang, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Chen, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zhao, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zhang, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
He, Q. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Qian, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wang, B. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1JOYryYdDsMvzt5-050XBwkOViBhWaLs-/view?usp=drivesdk">https://drive.google.com/file/d/1JOYryYdDsMvzt5-050XBwkOViBhWaLs-/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
