Cultivation at 6-10 C is an effective strategy to overcome the insolubility of recombinant proteins in Escherichia coli (Record no. 53995)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01689nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162438.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-19886 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Cultivation at 6-10 C is an effective strategy to overcome the insolubility of recombinant proteins in Escherichia coli |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Protein expression and purification, 82(2), p.297-301, 2012 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Protein expression in Escherichia coli at 15-25°C is widely used to increase the solubility of recombinant proteins. However, many recombinant proteins are insolubly expressed even at those low temperatures. Here, we show that recombinant proteins can be expressed as soluble forms by simply lowering temperature to 6-10°C without cold adapted chaperon systems. By using E. coli Rosetta-gami2(DE3), we obtained 1.8 and 0.9mg of Cryptopygus antarticus mannanase (CaMan)and cellulase (CaCel)from 1l culture grown at 6 and 10°C, respectively. Cultivation at 10°C also led to successful expression of EM3L7 (a lipase isolated from a metagenomic library)in a soluble form in E. coli BL21(DE3). Consequently, E. coli cultivation at 6-10°C is an effective strategy for overcoming a major hurdle of the inclusion body formation. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PROTEIN EXPRESSION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | E. COLI |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | EXTREMELY LOW TEMPERATURE |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Song, J. M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | An, Y. J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kang, M. H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lee, Y. H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cha, S. S. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/14VYn7JoWqJHay54p76fwt86tgSLKMWD_/view?usp=drivesdk">https://drive.google.com/file/d/14VYn7JoWqJHay54p76fwt86tgSLKMWD_/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-19886 | 25.06.2025 | 25.06.2025 | Documentos solicitados |