Efficient renaturation of inclusion body proteins denatured by SDS (Record no. 53997)

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fixed length control field 01701nam a2200229Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162438.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-19888
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245 10 - TITLE STATEMENT
Title Efficient renaturation of inclusion body proteins denatured by SDS
490 0# - SERIES STATEMENT
Volume/sequential designation BioChemical and biophysical Research Communications, 490(4), p.1250-1253, 2017
520 3# - SUMMARY, ETC.
Summary, etc. Inclusion bodies are often formed when the foreign protein is over expressed in Escherichia coli. Since proteins in inclusion bodies are inactive, denaturing and refolding of inclusion body proteins are necessary to obtain the active form. Instead of the conventional denaturants, urea and guanidine hydrochloride, a strong anionic detergent SDS was used to solubilize C-terminal His-tag form of ulvan lyase in the inclusion bodies. Solution containing SDS-solubilized enzyme were kept on ice to precipitate SDS, followed by SDS-KCl insoluble crystal formation to remove SDS completely. After removing the precipitate by centrifugation, the supernatant was applied to Ni-NTA column to purify His-tagged ulvan lyase. The purified protein showed a dimeric form and ulvan lyase activity, demonstrating that SDS-denatured protein was renatured and recovered enzyme activity. This simple method could be useful for refolding other inclusion body proteins.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element INCLUSION BODIES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element REFOLDING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SDS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ULVAN LYASE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name He, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ohnishi, K.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1xRXtnTZ5oAtSdRBbI7ygP4-VHEhSJPxA/view?usp=drivesdk">https://drive.google.com/file/d/1xRXtnTZ5oAtSdRBbI7ygP4-VHEhSJPxA/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-19888 25.06.2025 25.06.2025 Documentos solicitados