MARC details
| 000 -LEADER |
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| fixed length control field |
02995nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162439.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-19902 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Thidiazuron, a phenyl.urea cytokinin, inhibits ergosterol synthesis and attenuates biofilm formation of Candida albicans |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
World Journal of MicroBiology & Biotechnology, 38(12), p.1-14, 2022 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Candida albicans is a common human fungal pathogen that colonizes mucosa and develops biofilm in the oral cavity that causes oral candidiasis. It has been reported that cytochrome P450 enzyme (CYP51), a vital part of the ergosterol synthesis cascade, is associated with Candida infections and its biofilm formation. Thidiazuron, a phenyl-urea cytokinin, exhibits anti-senescence and elicitor activity against fungal infection in plants. However, how Thidiazuron impacts C. albicans biofilm formation is still uncertain. Here, we aimed to investigate the effects of a Thidiazuron against the growth and biofilm formation properties of C. albicans using in silico and in vitro experimental approaches. A preliminary molecular docking study revealed potential interaction between Thidiazuron and amino acid residues of CYP51. Further in vitro antifungal susceptibility test, scanning electron microscopy (SEM)and time kill analysis revealed the anti-fungal activity of Thidiazuron in both dose and time-dependent manner. Crystal violet staining, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT)reduction assay revealed 50 percent inhibition in C. albicans biofilm by Thidiazuron at concentrations 11 and 19 µM respectively. Acridine orange staining assay visually confirmed the biofilm inhibitory potential of Thidiazuron. The gene expression study showed that Thidiazuron treatment down regulated the expression of genes involved in ergosterol synthesis (ERG3, ERG11, ERG25), cell adhesion (ASL3, EAP1), and hyphae development (EFG1, HWP1, SAP5)in C. albicans. Wherease, the expression of negative transcription regulator of hyphae (NRG1)was upregulated (5.7-fold)by Thidiazuron treatment. Collectively, our data suggest that Thidiazuron is a robust antifungal compound and an outstanding biofilm inhibitor, which may promise further therapeutic development due to CYP51 binding and inhibition of ergosterol formation against C. albicans. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ANTI-FUNGAL |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
BIOFILM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CYP51 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CANDIDA ALBICANS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ERGOSTEROL |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
THIDIAZURON |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Harikrishnan, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Arayambath, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Jayaraman, V. K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Ekambaram, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Ahmed, E. A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Senthilkumar, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Thirugnanasambantham, K. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1r2t05xY8ILsAj4rrz6hdjw6xVgGL7krk/view?usp=drivesdk">https://drive.google.com/file/d/1r2t05xY8ILsAj4rrz6hdjw6xVgGL7krk/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
