A hydroponic cultivation system for rapid high-yield transient protein expressioin in Nicotiana plants under laboratory conditions (Record no. 54028)
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| fixed length control field | 02865nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162439.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-19922 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | A hydroponic cultivation system for rapid high-yield transient protein expressioin in Nicotiana plants under laboratory conditions |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of Southern Medical University, 32(6), p.772-777, 2012 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Objective: To develop a hydroponic Nicotiana cultivation system for rapid and high-yield transient expression of recombinant proteins under laboratory conditions. Methods: To establish the hydroponic cultivation system, several parameters were examined to define the optimal conditions for the expression of recombinant proteins in plants. We used the green fluorescent protein (GFP)and the geminiviral plant transient expression vector as the model protein/expression vector. We examined the impact of Nicotiana species, the density and time of Agrobacterium infiltration, and the post-infiltration growth period on the accumulation of GFP. The expression levels of GFP in Nicotiana leaves were then examined by Western blotting and ELISA. Results: Our data indicated that a hydroponic Nicotiana cultivation system with a light intensity of 9000 LX/layer, a light cycle of 16 h day/8 h night, a temperature regime of 28 degrees celsius; day/21 degrees celsius; night, and a relative humidity of 80 percent could support the optimal plant growth and protein expression. After agroinfiltration with pBYGFPDsRed.R/LBA4404, high levels of GFP expression were observed in both N. benthamiana and N. tobaccum (cv. Yuyan No.5)plants cultured with this hydroponic cultivation system. An optimal GFP expression was achieved in both Nicotiana species leaves 4 days after infiltration by Agrobacterium with an OD(600)of 0.8. At a given time point, the average biomass of N. tobaccum (cv. Yuyan No.5)was significantly higher than that of N. benthamiana. The leaves from 6-week-old N. benthamiana plants and 5-week-old N. tobaccum (cv. Yuyan No.5)plants could be the optimal material for agroinfiltration. Conclusion: We have established a hydroponic cultivation system that allows robust growth of N. benthamiana and N. tobaccum (cv. Yuyan No.5)plants and the optimal GFP expression in the artificial climate box. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | NICOTIANA PLANTS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | TRANSIENT EXPRESSION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HYDROPONIC CULTIVATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GREEN FLUORESCENT PROTEIN |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mo, Q. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mai, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yang, Z. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chen, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yang, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lai, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhou, X. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1xMB9pSMJwnd5QyVuiNqH-2mDmWsp8cM8/view?usp=drivesdk">https://drive.google.com/file/d/1xMB9pSMJwnd5QyVuiNqH-2mDmWsp8cM8/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-19922 | 25.06.2025 | 25.06.2025 | Documentos solicitados |