MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02588nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20250625162439.0 |
| 040 ## - CATALOGING SOURCE |
|---|
| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-19947 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
A Modified Method for Quantification of Cytokinin-Receptor Binding Using Isolated Plant Microsomes Enriched with Cognate Transmembrane Receptors |
| 490 0# - SERIES STATEMENT |
|---|
| Volume/sequential designation |
Russian Journal of Plant Physiology, 69(6), p.1-9, 2022 |
| 520 3# - SUMMARY, ETC. |
|---|
| Summary, etc. |
Cytokinin receptors are key proteins that trigger signaling of cognate classical phytohormones possessing a broad-spectrum biological action. Previously, we developed two test systems to analyze the ligand-binding properties of cytokinin receptors: a heterologous binding assay based on transformed bacteria E. coli, and, later, a more appropriate homologous assay based on microsomes from transiently transformed tobacco (Nicotiana benthamiana Domin.)leaves. In both cases, a genetic construct encoding one or another cytokinin receptor under study, was ectopically expressed. In the present work, we provide a versatile experimental validation for the homologous method and propose a modified (simplified)protocol for obtaining plant microsomes carrying transmembrane proteins to be studied, namely cytokinin receptors. In contrast to the original method, the simplified procedure makes it possible to avoid ultracentrifugation and to save reagents and time when obtaining a microsomal fraction. The microsomes isolated by the modified method were comparable in quantity and quality to a similar fraction obtained in a standard way, in both cases cytokinin specific binding by endogenous receptors was close to zero. Whichever way the microsomes were originally obtained (using an ultra- or preparative centrifuge), they precipitated equally well when subsequently centrifuged at 16?000 g in a benchtop microcentrifuge. All the experimental series allowed us to recommend a wide use of this plant-based binding method, in particular its simplified version, in studies of membrane-bound phytohormone receptors. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
NICOTIANA BENTHAMIANA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
AGROBACTERIUM TUMEFACIENS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
CYTOKININS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
RECEPTORS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
MICROSOMES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
MEMBRANE FRACTION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
PLANT-BASED BINDING ASSAY |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Savelieva, E. M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Lomin, S. N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Romanov, G. A. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1zz8591dUiPOBasAA554z6_Pzp5LhuEQv/view?usp=drivesdk">https://drive.google.com/file/d/1zz8591dUiPOBasAA554z6_Pzp5LhuEQv/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
