MARC details
| 000 -LEADER |
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| fixed length control field |
02580nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625162440.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-19984 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Hairy root cultures of Physalis minima L. - an alternative source of withaferin A production |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Plant Cell, Tissue and Organ Culture, 152, p.31-44, 2023 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Physalis minima L. is an important yet underutilised medicinal plant. It has many medicinal properties, including anticancer activity. This plant synthesizes a crucial steroidal lactone, withaferin A, with high medicinal value in addition to a number of alkaloids, steroids and phenolic compounds. We have established, for the first time, the hairy root culture (HRC)of this plant via explant cocultivation with the wild-type A4 strain of Agrobacterium rhizogenes to increase the production of the high-value steroidal lactone withaferin A. A maximum transformation efficiency of 83.49?±?2.45 percent was recorded with acetosyringone-treated (200 µM)bacterial culture. Genetic transformation was confirmed using primers specific to seven different genes. After screening several HR lines, five lines were selected based on their increased growth rate and high accumulation of withaferin A. Elicitation was applied to high-yielding root lines to further enhance the withaferin A accumulation. The effects of two biotic (chitosan and yeast extract)and three abiotic (salicylic acid, methyl jasmonate and aluminium chloride)elicitors on withaferin A accumulation were studied at different concentrations. While all the elicitors effectively increased withaferin A production, salicylic acid (4.14 mg l?1)induced an approximately 21-fold increase in withaferin A accumulation (19.081?±?0.058 mg g??1 DW)after 4 d of elicitation compared to the nonelicited culture (0.892?±?0.037 mg g??1 DW). These results suggest that the use of elicitors can tremendously increase the biosynthesis of withaferin A in this system; thus, the HRs of P. minima can be explored in the future for enhanced production of withaferin A. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
AGROBACTERIUM RHIZOGENES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ELICITATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
METHYL JASMONATE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SALICYLIC ACID |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
YEAST EXTRACT |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Halder, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Ghosh, B. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1azJoXO0gfe2CwP41feyFaHs9DkpjS6Qu/view?usp=drivesdk">https://drive.google.com/file/d/1azJoXO0gfe2CwP41feyFaHs9DkpjS6Qu/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
