Protoplast isolation and transient gene expression in different petunia cultivars (Record no. 54108)

MARC details
000 -LEADER
fixed length control field 02129nam a2200277Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162441.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20003
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Protoplast isolation and transient gene expression in different petunia cultivars
490 0# - SERIES STATEMENT
Volume/sequential designation Protoplasma, 260(1), p.271-280, 2023
520 3# - SUMMARY, ETC.
Summary, etc. The protocol optimized for Petunia hybrida cv. Mirage Rose produced high protoplast yields in 3 out of other 11 cultivars (Damask White, Dreams White, and Opera Supreme White). Factors optimized in the protoplast transfection process showed that the best transfection efficiency (80 percent)was obtained using 2.5?×?105 protoplast density, 40 percent polyethylene glycol (PEG)concentration, 10 µg plasmid DNA, and 15 min of transfection time. Assessing the usability of the protocol for other cultivars (Damask White, Dreams White, and Opera Supreme White), a reasonable protoplast transfection efficiency (?50 percent)was observed in the cultivars Dreams White and Opera Supreme White, with lower efficiency (?50 percent)observed in the cv. Damask White. The transient expression of enhanced green fluorescent protein (eGFP)in the nucleus of the transfected protoplasts of all cultivars was confirmed using PCR. This system could be valuable for genome editing of unwanted genes in petunias using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9)technology. Furthermore, it could contribute to other studies on protein subcellular localization, protein-protein interactions, and functional gene expression in the petunias.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element EGFP
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PCR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PETUNIA CULTIVARS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PROTOPLAST ISOLATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSFECTION EFFICIENCY
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kang, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Naing, A. H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Park, S. K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chung, M. Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kim, C. K.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1nSVwM9LaLQmmyulb-ZqieX4oRDby5_8p/view?usp=drivesdk">https://drive.google.com/file/d/1nSVwM9LaLQmmyulb-ZqieX4oRDby5_8p/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-20003 25.06.2025 25.06.2025 Documentos solicitados