PIN5 is involved in regulating NH4 + efflux and primary root growth under high.ammonium stress via mediating intracellular auxin transport (Record no. 54111)
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| 000 -LEADER | |
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| fixed length control field | 02443nam a2200301Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162441.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-20006 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | PIN5 is involved in regulating NH4 + efflux and primary root growth under high.ammonium stress via mediating intracellular auxin transport |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Plant and Soil, 10.1007/s11104-023-05869-z, 2023 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Background and Aims Ammonium (NH4+)is an important nitrogen (N)source in many ecosystems and agricultural systems but excessive NH4 + is toxic to root growth and development, especially when NH4 + is the sole N source. Previous studies have shown that polar auxin (indole-3-acetic acid, IAA)transport mediated by PIN2 and AUX1 is critical for maintaining lateral root development under high- NH4 + stress. However, the regulation of subcellular IAA homeostasis under high-NH4 + stress has remained unclear. Methods Knockout mutants for the intracelular IAA transporter PIN5 and the plasma-membrane H+- ATPases AHA1 and AHA2 were used, and primary root length, transmembrane H+ fluxes, and NH4 + fluxes in the roots were determined. Results We show that high NH4 + disturbs the subcellular IAA homeostasis by upregulating the expression of PIN5. Knockout of PIN5 resulted in elevated cytoplastic IAA accumulation and reduced NH4 + efflux under high-NH4 + stress. Furthermore, we show that NH4 + treatment promotes H+ efflux at the root elongation zone, in turn promoting NH4 + efflux, with the involvement of PIN5-mediated intracelular IAA transport. Moreover, stabilization of rhizosphere pH reduced NH4 + efflux and promoted primary root growth in the pin5 mutant under high NH4 +. Conclusion Our findings provide a mechanistic explanation for the role of subcellular IAA homeostasis in response to high-NH4 + stress through the coordinated regulation of NH4 + efflux and H+ efflux. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PIN5 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | AMMONIUM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | INTRACELLULAR AUXIN HOMEOSTASIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | H+ FLUXES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | NH4+FLUXES |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Di, D. W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wu, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ma, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Li, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kronzucker, H. J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Shi, W. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1aaL3YfnydFZ3Jr3hJWpRzUor9pbh6cfF/view?usp=drivesdk">https://drive.google.com/file/d/1aaL3YfnydFZ3Jr3hJWpRzUor9pbh6cfF/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-20006 | 25.06.2025 | 25.06.2025 | Documentos solicitados |