Comprehensive in vitro polymer type, concentration, and size correlation analysis to microplastic toxicity and inflammation (Record no. 54144)

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control field MX-MdCICY
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control field 20250625162441.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20039
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Title Comprehensive in vitro polymer type, concentration, and size correlation analysis to microplastic toxicity and inflammation
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Volume/sequential designation Science of The Total Environment, 854, p.158731, 2023
520 3# - SUMMARY, ETC.
Summary, etc. The ubiquitous nature of microplastic particles (MP)is a growing environmental and ecological concern due to their impact on aquatic and terrestrial systems and potentially on human health. The potential impact on human health may be due to MP daily exposure by several routes, but little is known about the cellular effects. Previous in vitro and in vivo studies have described inflammation, oxidative stress, and metabolic disruption upon plastic exposure, while the effect of individual plastic parameters is not fully unraveled. To this end, we investigated plastic exposure to different polymer types, sizes, and concentrations in three human cell lines (A549, HEK293, and HeLa). Particles were polystyrene (PS)or polymethylmethacrylate (PMMA)in three sizes and concentrations, and amine-modified PS served as positive control. After MP size validation using dynamic light scattering, a high-throughput high-content imaging-based and algorithm-driven multi-z-stack analysis was established to quantify intracellular fluorescent particle accumulation in 3D objects and cell maximum intensity projections. MP uptake correlated with concentration and for PS with size (1.040 ?m), while for PMMA it was maximal for 400 nm MP. Uptake increased in HEK cells independent of MP parameters. Except for positive controls, no major effect on metabolic activity, viability, and cell cycle was observed, while intracellular thiol content and cytokine secretion were affected to a considerable extent. Interestingly, particle uptake was correlated significantly with particle size and concentration, underlining the dependence of MP parameters on biological effects.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Da Silva Brito, W. A.
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Personal name Singer, D.
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Personal name Miebach, L.
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Personal name Saadati, F.
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Personal name Wende, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Schmidt, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bekeschus, S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/12sSbHZ-f0U6yDGUCUjnt6LZOkU5lIqVA/view?usp=drivesdk">https://drive.google.com/file/d/12sSbHZ-f0U6yDGUCUjnt6LZOkU5lIqVA/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-20039 25.06.2025 25.06.2025 Documentos solicitados