Chloroplast structure and DNA methylation polymorphisms in an albino mutant of wheat (Triticum aestivum)cv. Xinong 1376 (Record no. 54233)

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fixed length control field 03043nam a2200289Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162443.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20129
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245 10 - TITLE STATEMENT
Title Chloroplast structure and DNA methylation polymorphisms in an albino mutant of wheat (Triticum aestivum)cv. Xinong 1376
490 0# - SERIES STATEMENT
Volume/sequential designation Crop and Pasture Science, 69(4), p.362-373, 2018
520 3# - SUMMARY, ETC.
Summary, etc. DNA methylation plays an important role in regulating plant development, including organ and tissue differentiation, which may determine variations in agronomic traits. However, no reports exist for the regulation of leaf colour in wheat. The present study investigated the chloroplast structure and epigenetic mechanisms regulating leaf colour in an albino mutant of wheat (Triticum aestivum L.)cv. Xinong 1376. Structural analysis was performed by scanning and transmission electron microscopy, and epigenetic modifications were detected by methylation-sensitive amplification polymorphism (MSAP)analysis. Mesophyll cells of green leaves showed a well-ordered arrangement and they were filled with chloroplasts with intact lamellar structures and thylakoid membranes. By contrast, mesophyll cells of red and white leaves were disorganised and contained only a few plastids or chloroplasts with no lamellar structures or thylakoid membranes. Comparison of MSAP profiles revealed that white or red leaves had higher levels of cytosine methylation and showed changes in polymorphic loci compared with green leaves (4.35 percent and 4.10 percent, respectively). We sequenced 150 DNA fragments that were differentially displayed in MSAP patterns of white or red and green leaves of the Xinong 1376 albino mutant. A further BLAST search of 77 cloned sequences located them in coding regions. Most of these sequences were found to be involved in processes such as signal transduction, transcription regulation, post-transcriptional processing, DNA modification and repair, transport, biosynthesis of cellulose, photosynthesis, protein ubiquitination, stress responses, and retroposition. Expression analysis demonstrated a decrease in the transcription of two methylated genes, psaA and psbD, which are involved in the photosystem. Although the DNA methylation changes and leaf colour changes were not directly associated, these results may indicate that methylation of specific genes is an active and rapid epigenetic response to variation of leaf colour in the Xinong 1376 albino mutant, further elucidating the mechanism of variation in leaf colour.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ALBINISM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LEAF
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element METHYLATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHOTOSYNTHESIS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Song, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tang, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ke, X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Guo, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhang, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wang, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhang, G.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1GjyBRnk3U_sgDbq_aGWrNv-sIbiSnMUO/view?usp=drivesdk">https://drive.google.com/file/d/1GjyBRnk3U_sgDbq_aGWrNv-sIbiSnMUO/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-20129 25.06.2025 25.06.2025 Documentos solicitados