Image-based quantification of Arabidopsis thaliana stomatal aperture from leaf images (Record no. 54261)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02336nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162443.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-20158 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Image-based quantification of Arabidopsis thaliana stomatal aperture from leaf images |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | bioRxiv, 10.1093/pcp/pcad018 , 2023 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The quantification of stomatal pore size has long been a fundamental approach to understand the physiological response of plants in the context of environmental adaptation. Automation of such methodologies not only alleviates human labor and bias, but also realizes new experimental research methods through massive analysis. Here, we present an image analysis pipeline that automatically quantifies stomatal aperture of Arabidopsis thaliana leaves from brightfield microscopy images containing mesophyll tissue as noisy backgrounds. By combining a YOLOX-based stomatal detection submodule and a U-Net-based pore segmentation submodule, we achieved 0.875 mAP50 (mean average precision; stomata detection performance)and 0.745 IoU (intersection of union; pore segmentation performance)against images of leaf discs taken with a brightfield microscope. Moreover, we designed a portable imaging device that allows easy acquisition of stomatal images from detached/undetached intact leaves on-site. We demonstrated that this device in combination with fine-tuned models of the pipeline we generated here provides robust measurements that can substitute for manual measurement of stomatal responses against pathogen inoculation. Utilization of our hardware and pipeline for automated stomatal aperture measurements is expected to accelerate research on stomatal biology of model dicots |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ARABIDOPSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ENVIRONMENTAL RESPONSE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | IMAGE ANALYSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PLANT PHENOTYPING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PLANT-MICROBE INTERACTIONS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | STOMATA |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Takagi, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hirata, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Aihara, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hayashi, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Aihara, M. M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ando, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Toda, Y. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1oK8NJd8lL-TTlqNhSgE9a9nXuitibVjW/view?usp=drivesdk">https://drive.google.com/file/d/1oK8NJd8lL-TTlqNhSgE9a9nXuitibVjW/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-20158 | 25.06.2025 | 25.06.2025 | Documentos solicitados |