1 In Vivo Detection of Double-Stranded RNA by dRBFC Assay; 2 Developing Rice Mutants Using CRISPR/Cas9-Based Genome Editing Technology; 3 Microscale Thermophoresis Assay: A Powerful Method to Quantify Protein-Nucleic Acid and Protein-Protein Interactions; 4 Transient Expression-Mediated Gene Silencing in Plants and Suppression of Gene Silencing with Viral Suppressors; 5 Isolation and Transfection of Plant Mesophyll Protoplasts for Virology Research; 6 Split-Luciferase Complementation for Analysis of Virus-Host Protein Interactions; 7 Monitoring Virus Intercellular Movement from Primary Infected Cells to Neighboring Cells in Plants; 8 Simultaneous Determination and Subcellular Localization of Protein-Protein Interactions in Plant Cells Using Bimolecular Fluorescence Complementation Assay; 9 A Powerful Method for Studying Protein-Protein Interactions in Plants: Coimmunoprecipitation (Co-IP)Assay; 10 Membrane and Nuclear Yeast Two-Hybrid Systems; 11 Tag-Based Pull-Down Assay; 12 Purification of Plasmodesmata-Enriched Fraction for Proteomic Analyses; 13 Purification and Proteomics Analysis of Phloem Tissues from Virus-Infected Plants; 14 Rapid and Specific Purification of Argonaute-Small RNA Complexes from Rice for Slicer Activity; 15 Silencing Specific Genes in Plants Using Virus-Induced Gene Silencing (VIGS)Vectors; 16 A Versatile Expression Platform in Insects and Cereals Based on a Cytorhabdovirus; 17 Production of Virus-Free Chrysanthemum (Chrysanthemum morifolium Ramat)by Tissue Culture Techniques; 18 Long-Term Preservation of Plant Viruses in Cryopreserved; 19 Establishment of White-Backed Planthopper Cell Lines; 20 Biolistic Inoculation of Fruit Trees with Full-Length Infectious cDNA Clones of RNA Viruses; 21 Purification of Total RNAs and Small RNAs from Fruit Tree Leaf Tissues; 22 Diagnosis of Viral Diseases Using Deep Sequencing and Metagenomics Analyses; 23 Immunocapture-Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay for Detection of Plant RNA Viruses; 24 Detection of Tomato Spotted Wilt Virus (TSWV)Infection in Plants Using DAS-ELISA and Dot-ELISA; 25 Tube-Capture (TC)RT-PCR and Multiplex RT-PCR for Diagnosis and Characterization of Viruses in Fruit Trees.; 26 Detection of Cucumber green mottle mosaic virus (CGMMV)in Cucurbitaceous Crop Seeds by RT-PCR; RNA In Situ Hybridization of Detecting Cucumber Mosaic Virus in Shoots of Nicotiana benthamiana Plants; 28 Transmission Electron Microscopic Methods for Plant Virology; (Record no. 54361)