Metabolic changes in hairy root cultures of Centella asiatica treated with methyl-jasmonate and coronatine: a 1H-NMR-based metabolomics approach (Record no. 54390)
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| fixed length control field | 02855nam a2200301Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162446.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-20291 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Metabolic changes in hairy root cultures of Centella asiatica treated with methyl-jasmonate and coronatine: a 1H-NMR-based metabolomics approach |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | In Vitro Cellular & Developmental Biology-Plant, 59, p.179-196, 2023 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Hairy root cultures of Centella asiatica-based coupled with exogenous application of methyl-jasmonate and coronatine are suitable bioproduction platforms to overproduce this plant-occurring high-valuable metabolite. These two molecules orchestrate cellular responses in metabolism that have not been yet deeply explored in hairy roots of this plant. The exploration of metabolic changes is crucial since the biotechnological system productivity depends on the knowledge of the biosynthetic machinery of the culture. To understand how methyl-jasmonate and coronatine cause changes in the metabolism of hairy roots of C. asiatica, the metabolic responses to these molecules were monitored through a nuclear magnetic resonance time-series metabolomics approach. The acquired data was analyzed to perform untargeted and targeted metabolomics approaches. Multivariate data analysis statistical models revealed that coronatine caused an early and superior reprogramming effect on the metabolome compared to methyl-jasmonate. We identified 40 compounds from primary and specialized metabolism. The relative production kinetics of these metabolites in the two treatments and their possible links in the metabolic pathways of the plant were discussed. We were able to observe that in coronatine-treated cultures, phenolic specialized pathways were activated 8 d after treatment exposure while the pool of carbohydrates and nitrogen-carrier amino acids were consumed at early stages. This evidenced the clear and high reprogramming effect in the metabolism of C. asiatica hairy roots in response to coronatine. These findings provide important insights on the metabolic alterations caused by methyl-jasmonate and coronatine and on the possibility to redirect the metabolism of hairy root cultures of C. asiatica. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | C. ASIÁTICA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HAIRY ROOTS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | 1H-NMR METABOLOMICS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CORONATINE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | METABOLISM REGULATION |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Garcia-Baeza, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Alcalde, M. A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Grovel, O. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Balderas-Renteria, I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Villa-Ruano, N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Velázquez-Ponce, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ramirez-Estrada, K. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1MyiLbFc0tUzrxG9fe5qnK0soUdekz71R/view?usp=drivesdk">https://drive.google.com/file/d/1MyiLbFc0tUzrxG9fe5qnK0soUdekz71R/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-20291 | 25.06.2025 | 25.06.2025 | Documentos solicitados |