An improved procedure for the isolation and purification of protoplasts from carrot suspension culture (Record no. 54432)

MARC details
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003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162447.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20336
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245 10 - TITLE STATEMENT
Title An improved procedure for the isolation and purification of protoplasts from carrot suspension culture
490 0# - SERIES STATEMENT
Volume/sequential designation Planta, 147, p.283-286, 1980
520 3# - SUMMARY, ETC.
Summary, etc. A procedure is reported for the rapid and highly reproducible isolation of protoplasts from carrot suspension culture. The method utilizes Onozuka R 10 cellulase which has been purified by chromatography on Sephadex G75. Protoplast isolation, using this procedure, is quantitative and complete within 1 to 1.5 h. Intact protoplasts were separated from broken ones and other cellular debris by application of a polyethylene glycol/dextran two-phase system. The protoplasts isolated in this manner lack any detectable cell wall and are greater than 95percent viable when assayed using fluorescein diacetate. It is concluded that such protoplasts are highly suitable for biochemical studies.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELLULASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DAUCUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PROTOPLAST
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TISSUE CULTURE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Slabas, A. R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Powell, A. J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lloyd, C. W.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1EllPGsU_P43rHstp9HdAlN5g3htjl7IZ/view?usp=drivesdk">https://drive.google.com/file/d/1EllPGsU_P43rHstp9HdAlN5g3htjl7IZ/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-20336 25.06.2025 25.06.2025 Documentos solicitados