Natural variations in Crocus sativus lycopene epsilon cyclase (CstLcyE)alter carotenoid/apocarotenoid content and stress tolerance (Record no. 54493)

MARC details
000 -LEADER
fixed length control field 02330nam a2200277Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625162448.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20398
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Natural variations in Crocus sativus lycopene epsilon cyclase (CstLcyE)alter carotenoid/apocarotenoid content and stress tolerance
490 0# - SERIES STATEMENT
Volume/sequential designation Environmental and Experimental Botany, 214, p.105457, 2023
520 3# - SUMMARY, ETC.
Summary, etc. Crocus sativus is an economically and pharmaceutically important crop. The dried stigmas of C. sativus flowers constitute saffron, the only commercial source of apocarotenoids like crocin, picrocrocin, and safranal. Some elite clones of C. sativus with higher crocin content were identified from plant populations over three years. Sequencing approach demonstrated that mutations are present in CstLycE. The C. sativus apocarotenoids are formed by cleavage of ?-branch carotenoids. Cyclization of lycopene is the key branch point in carotenoid/apocarotenoid pathway. While lycopene ?-cyclase converts lycopene into ?-carotene, combined action of ? and ?-cyclases forms ?-carotene. Towards this, the mutant populations with critical mutations in CstLycE result in loss of function, and hence the metabolic flux was diverted towards ?-branch. In-silico analysis and site-directed mutagenesis confirmed the critical nature of these mutations. To ascertain the role of CstLycE, transient over-expression and RNAi lines of CstLcyE in C. sativus were generated, and we observed enhanced ?-branch carotenoid and apocarotenoid content in RNAi lines. The effect of CstLcyB and CstLcyE was compared, and CstLcyE RNAi lines had a more profound impact on apocarotenoid accumulation than CstLcyB over-expression lines. CstLcyE-RNAi plants also accumulated more abscisic acid and were more tolerant to abiotic stresses.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ABIOTIC STRESS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CROCUS SATIVUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LYCOPENE CYCLASES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ENZYME ACTIVITY
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mir, J. A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yadav, A. K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Singh, D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gani, U.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Misra, P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ashraf, N.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/16Yqa99e3QNp3V25ydlkYdO0bHDCqfik5/view?usp=drivesdk">https://drive.google.com/file/d/16Yqa99e3QNp3V25ydlkYdO0bHDCqfik5/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-20398 25.06.2025 25.06.2025 Documentos solicitados